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Merck
CN

AB5896

P2X3受体抗体,疼痛

serum, Chemicon®

别名:

P2X3抗体

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
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产品名称

P2X3受体抗体,疼痛, serum, Chemicon®

biological source

guinea pig

conjugate

unconjugated

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, rat, mouse

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

Gene Information

human ... P2RX3(5024)

Application

免疫组化:1:1,000。

免疫细胞化学:1:1,000。

最佳工作稀释度必须由最终用户进行确定。

AB5896应用说明

免疫组织化学

雄性SD大鼠(体重100-150g)用戊巴比妥钠麻醉,并通过升主动脉灌注:1)50 mL不含Ca2+的台氏液,然后2)福尔马林-苦味酸固定剂(4%多聚甲醛和0.4%苦味酸溶于0.16 M磷酸盐缓冲液,pH 6.9)和3)10%蔗糖的PBS溶液作为冷冻保护剂。快速解剖组织,并在含有10%蔗糖的0.1 M磷酸盐缓冲液(pH值7.4)中保存过夜。将载玻片固定的组织切片与封闭缓冲液在室温下培养1小时。将一抗在封闭缓冲液中稀释至适当的工作稀释度。去除封闭缓冲液,然后将载玻片与AB5896(1:1000)在2-8°C下孵育18-24小时。在PBS中冲洗3次后,将切片与Cy3偶联的二抗在室温下培养60分钟。固定在含有0.1%对苯二胺的PBS和甘油(1:3)的混合物中后,用Nikon Microphot-SA落射荧光显微镜检查切片。

免疫细胞化学

对2X3转染的细胞进行间接免疫荧光处理。去除培养基,用无血清培养基轻轻洗涤细胞3次。固定后,如上所述对细胞进行间接免疫荧光处理。
研究子类别
神经递质&受体

神经炎症&疼痛
研究类别
神经科学
这种P2X3受体抗体(疼痛)经验证可用于IC、IH检测P2X3受体。

Biochem/physiol Actions

P2X3受体。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

Immunogen

15个氨基酸的肽,对应于大鼠P2X3受体蛋白羧基末端的氨基酸383-397。

对照肽: 货号AG356

Physical form

血清。液体。含0.05%叠氮化钠。

Preparation Note

以未稀释的等分试样保存于-20°C下6个月。应避免反复冻/融循环。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

10 - Combustible liquids

wgk

WGK 1


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Osteoarthritis (OA) is a degenerative and progressive disease characterized by cartilage breakdown and by synovial membrane inflammation, which results in disability, joint swelling, and pain. The purinergic P2X3 and P2X2/3 receptors contribute to development of inflammatory hyperalgesia, participate in arthritis
J L Saloman et al.
Neuroscience, 232, 226-238 (2012-12-04)
Musculoskeletal pain conditions, particularly those associated with temporomandibular disorders (TMD) affect a large percentage of the population. Identifying mechanisms underlying hyperalgesia could contribute to the development of new treatment strategies for the management of TMD and other muscle pain conditions.
Zhiyong Chen et al.
Cells, 11(15) (2022-07-28)
The purinergic system plays an important role in pain transmission. Recent studies have suggested that activation of P2-purinergic receptors (P2Rs) may be involved in neuron-satellite glial cell (SGC) interactions in the dorsal root ganglia (DRG), but the details remain unclear.
Z-J Wang et al.
Anatomy and embryology, 207(4-5), 363-371 (2003-11-19)
Intraganglionic laminar endings (IGLEs) represent the most prominent vagal afferent terminal structures throughout the gastrointestinal tract. They are most prominent in the esophagus and stomach, but can be found down to the distal colon. Their role as mechanosensors as proposed
Jiayin Li et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 40(2), 297-310 (2019-11-21)
Pancreatitis-associated proteins (PAPs) display multiple functions in visceral diseases. Previous studies showed that the expression level of PAP-I was low in the DRG of naive rats but was de novo expressed after peripheral nerve injury. However, its role in neuropathic

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