Anti-Dab1 Antibody

Dab1 is a protein arising from the mouse gene dab, that is related to the Drosophila gene ′disabled′. Mutations in the mouse dab gene may result in the ′scrambler′ and ′yotari′ phenotypes. Dab encodes a phosphoprotein that binds non-receptor tyrosine kinases and has been implicated in neuron development. Dab1 is expressed in neuronal populations exposed to reelin, as determined by in situ hybridization to embryonic mouse brain tissue. Dab1 may function as a signaling molecule that regulates positioning in the developing brain. Cloning of human DAB1 and sequence determinations suggests a 96% homology to the mouse sequence. Very low density lipoprotein receptors (VLDLR) and apolipoprotein E receptor-2 (APOER2) both bind Dab1 on their cytoplasmic tails. Dab1 expression is upregulated in knockout mice lacking both Vldlr and Apoer2 genes.

On western blots of mouse brain extracts, the antibody recognizes a band migrating at approximately 80 kDa corresponding to Dab1. No cross reactivity to human or mouse Dab2.

Highly purified GST-fusion protein corresponding to residues 400-555 of mouse Dab1.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Signaling Neuroscience

This Anti-Dab1 Antibody is validated for use in IC, IH, IP, WB for the detection of Dab1.

Western blotting: 1:1,000-1:5,000

Immunocytochemistry: 1:500-1:1,000

Immunohistochemistry: 1:5,000-1:20,000 on cryofixed tissues

Immunoprecipitation

Optimal working dilutions must be determined by the end user.

80 kDa

Replaces: MABS167

Serum, delipidated and defibrinated

Delipidated and defibrinated Serum in 0.02M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide.

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Control
Brain tissue

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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