Anti-Syntaxin 1 Antibody

Recognizes Syntaxin 1A and Syntaxin 1B. The epitope does not share homology with any other known proteins.

GST fusion protein corresponding to a cytoplasmic part of rat Syntaxin 1A (Accession number P32851) (Bennet et al. 1992; Inoue & Akagawa 1992).

Research Category
Neuroscience

Research Sub Category
Synapse & Synaptic Biology

This Anti-Syntaxin 1 Antibody is validated for use in WB, IH for the detection of Syntaxin 1.

Western blot: 1:700-1:1,250 (0.8-1.5 g/mL) using ECL on rat brain membranes.

Immunohistochemistry: AB5820 is directed against an intracellular epitope. Thus, a procedure including permeabilization of cells with 0.2% Triton X 100 is recommended. It is recommended that you start with a working concentration of 30 μg/mL (1:33).

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 50 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 mL of PBS. For positive control, in Western blot using 20 ng of protein per Minigel lane. For negative control, preincubate 5 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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