推荐产品
生物来源
rabbit
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
mouse, rat, human
制造商/商品名称
Chemicon®
技术
immunohistochemistry: suitable
western blot: suitable
UniProt登记号
运输
dry ice
靶向翻译后修饰
unmodified
基因信息
human ... BDNF(627)
一般描述
脑源性神经营养性因子(UniProt P23560;也称为Abrineurin,BDNF,Neurotrophin)由人的BDNF(也称为BULN2)基因(基因ID 627)编码。BDNF是神经营养因子(也称为神经营养因子)NGF家族的成员,是中枢和外周神经系统中特定神经元亚群的分化和存活所必需的。神经营养因子家族由至少四种蛋白质组成,包括NGF,BDNF,NT3和NT4/5。BDNF通过高亲和细胞表面受体gp145/trkB发出信号来介导其神经营养特性。BDNF最开始是由一个信号肽(a.a.1-18)和前肽(aa19-128)序列产生的,去除该序列会产生成熟的BDNF(a.a.129-247)。
特异性
该多克隆抗体可检测前体BDNF和前BDNF,以及前体BDNF/前BDNF衍生的含有前肽序列的N末端片段(a.a.19-128),但不检测含前肽序列的成熟BDNF或C末端片段。与其他神经营养因子原无交叉反应。
免疫原
对应于人BDNF前肽序列内区域的KLH偶联的线性肽。
表位:前肽。
应用
抗脑源性神经营养因子抗体,pro是用于免疫组化和蛋白质印迹的抗体proBDNF。
研究子类别
神经化学&神经营养因子
神经炎症&疼痛
神经化学&神经营养因子
神经炎症&疼痛
研究类别
神经科学
神经科学
蛋白质印迹:1:2,000
免疫组化:1:2,000
最佳工作稀释度必须由最终用户进行确定。
免疫组化:1:2,000
最佳工作稀释度必须由最终用户进行确定。
目标描述
观察值约35 kDa(糖基化前体/前BDNF),约50-65 kDa(糖基化前体/前BDNF二聚体)和约26-28 kDa(非糖基化前体/前BDNF或糖基化前体/前BDNF N末端片段)。由于糖基化和/或二聚化,目标条带似乎大于计算的分子量27.82/28.87/29.82/37.12/31.12 kDa(人前体-BDNF亚型1/2/3/4/5)和25.77/26.82/27.77/35.07/29.06 kDa(人前-BDNF亚型1/2/3/4/5)。某些裂解液可能会出现未表征的条带。
外形
从10 mM PBS中进行冻干。用20 μL无菌蒸馏水复溶。
储存及稳定性
在接收之日起,将冻干物料在-20°C下保存长达6个月。复溶后以未稀释状态在-20°C下保存长达 6个月。应避免反复冻/融循环。可添加(1:1)甘油(ACS级或更优级)以获得额外的稳定性。
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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