生物来源
rabbit
质量水平
抗体形式
serum
抗体产品类型
primary antibodies
克隆
polyclonal
种属反应性（根据同源性预测）
mammals
制造商/商品名称
Chemicon®
技术
ELISA: suitable
immunohistochemistry: suitable
适用性
not suitable for immunohistochemistry (Paraffin)
运输
wet ice
靶向翻译后修饰
unmodified
特异性
Citrulline in glutaraldehyde fixed tissues or cells.
The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity ratio
Citrulline-G-BSA 1
Arginine-G-BSA 1/>100,000
Glutamine-G-BSA 1/>100,000
Ornithine-G-BSA 1/>100,000
Abbreviations:
(G) Glutaraldehyde
(BSA) Bovine Serum Albumin
The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity ratio
Citrulline-G-BSA 1
Arginine-G-BSA 1/>100,000
Glutamine-G-BSA 1/>100,000
Ornithine-G-BSA 1/>100,000
Abbreviations:
(G) Glutaraldehyde
(BSA) Bovine Serum Albumin
免疫原
Citrulline-Glutaraldehyde-BSA
应用
Anti-Citrulline Antibody is a Rabbit Polyclonal Antibody for the detection of Citrulline & has been validated in ELISA & IHC.
Immunohistochemistry: 1:1,000-1:2,500 glutaraldehyde required for reactivity. (0.5%-2.5% is typical; fluorescence detection is not recommended. Blocking is with 3% normal serum with 0.1-0.2% triton X-100 usually. Primary antibody is diluted only in TBS or PBS with out blocking or triton X-100. Incubations are usually 3 hours 37C or 4C overnight. Detection is by ABC or PAP for best results. Not recommended for paraffin embedded tissues.
ELISA: 1:2500-1:5000
Optimal working dilutions must be determined by the end user.
ELISA: 1:2500-1:5000
Optimal working dilutions must be determined by the end user.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
外形
Rabbit antiserum. Liquid with 0.05% sodium azide
储存及稳定性
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Charlotte Thålin et al.
Journal of thrombosis and haemostasis : JTH, 18(10), 2732-2743 (2020-07-13)
Recent data propose a diagnostic and prognostic capacity for citrullinated histone H3 (H3Cit), a marker of neutrophil extracellular traps (NETs), in pathologic conditions such as cancer and thrombosis. However, current research is hampered by lack of standardized assays. We aimed
Priyanka Sharma et al.
Molecular cell, 73(1), 84-96 (2018-11-26)
The post-translational modification of key residues at the C-terminal domain of RNA polymerase II (RNAP2-CTD) coordinates transcription, splicing, and RNA processing by modulating its capacity to act as a landing platform for a variety of protein complexes. Here, we identify
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