推荐产品
产品名称
抗神经丝H抗体, Chemicon®, from chicken
生物来源
chicken
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
polyclonal
种属反应性
rat, pig, bovine, feline, human, mouse
制造商/商品名称
Chemicon®
技术
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
NCBI登记号
UniProt登记号
运输
dry ice
靶向翻译后修饰
unmodified
基因信息
bovine ... Nefh(528842)
cat ... Nefh(101087272)
human ... NEFH(4744)
mouse ... Nefh(380684)
pig ... Nefh(100156492)
rat ... Nefh(24587)
特异性
识别神经丝重(NF-H)。该抗体染色神经元细胞体(尤其是轴突)中清晰定义的细胞质丝。
免疫原
纯化的牛NF-H。
应用
抗神经丝H抗体是针对神经丝的抗体,可用于IC、IH、IH(P)& WB。
蛋白质印迹:使用ECL为1:1,000-1:10,000识别200-220 kDa的条带。
免疫组化:在冷冻或石蜡包埋的组织切片上为1:1,000-1:10,000。建议的固定剂为4%多聚甲醛。
免疫细胞化学:在组织培养中的细胞上为1:1,000-1:10,000,细胞在3.7%福尔马林中固定一分钟,在-20°C甲醇中固定一分钟。
最佳工作稀释度必须由最终用户进行确定。
免疫组化:在冷冻或石蜡包埋的组织切片上为1:1,000-1:10,000。建议的固定剂为4%多聚甲醛。
免疫细胞化学:在组织培养中的细胞上为1:1,000-1:10,000,细胞在3.7%福尔马林中固定一分钟,在-20°C甲醇中固定一分钟。
最佳工作稀释度必须由最终用户进行确定。
目标描述
200kda
外形
在含有5mM叠氮化钠的PBS中纯化的鸡IgY。
形式:纯化
分析说明
对照
阳性对照:脑。
阴性对照:肾,肝。
阳性对照:脑。
阴性对照:肾,肝。
其他说明
浓度:请参考批次特异性浓缩物的分析证书。
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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