Anti-AANAT Antibody

Recognizes AANAT (Arylalkylamine N-acetyltransferase). The antibody specifically recognizes the phosphorylated N-terminal PKA site of AANAT (pT31) (Ganguly et al. 2005). The antibody reacts with natural and recombinant protein.

Synthetic peptide (phosphorylated) corresponding to amino acids near the N-terminous of rat AANAT conjugated to BSA. {Ganguly, S et al, 2005}.

Anti-AANAT Antibody is an antibody against AANAT for use in WB.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Western Blot: 1:10,000-1:20,000 using ECL. Reacts with the ~23 kDa AANAT protein. Some reactivity may also be seen at ~97 kDa. Suggested blocking buffer is PBS with 10% non fat dry milk and 0.2% Tween-20. Suggested dilution buffer is PBS with 1 mg/mL BSA fraction V. Suggested incubation time is overnight at room temperature (~23°C).

Optimal working dilutions must be determined by the end user.

Liquid rabbit serum containing no preservative.

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Control
POSITIVE CONTROL:

Night adapted rat pineal gland. Expression is light sensitive; highest levels are obtained from animals at night.

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