Anti-Acid Sensitive Ion Channel 2 Antibody, neuronal

Recognizes ASIC2. The epitope used for the immunogen is specific for ASIC2 and is not similar to any other known protein. The epitope is not specific for ASIC2b (MDEG2) splice variant.

SPECIES REACTIVITIES: It is expected that the antibody will also react with mouse due to immunogen sequence homology (100%). Reactivity with other species has not yet been tested.

Epitope: neuronal

Highly purified peptide corresponding to residues 2-18 of rat or human ASIC2 (Accession Q16515).

Anti-Acid Sensitive Ion Channel 2 Antibody, neuronal is an antibody against Acid Sensitive Ion Channel 2 for use in WB.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Western blotting: 1:200 using ECL on rat brain membranes.

Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.

Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA, 5% sucrose and 0.025% sodium azide. Reconstitute with 50 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).

Maintain lyophilized material at -20°C for up to 6 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The antigen should be reconstituted with 100 μL of deionized water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.