生物来源
sheep
质量水平
抗体形式
affinity purified immunoglobulin
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
mouse, rat
制造商/商品名称
Chemicon®
技术
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
UniProt登记号
运输
dry ice
靶向翻译后修饰
unmodified
基因信息
human ... TPM3(7170)
特异性
识别原肌球蛋白5NM1和5NM2。
免疫原
对应于来自原肌球蛋白γ基因的9d外显子的合成肽。
表位:5NM1和5NM2。
应用
使用经验证可用于WB、IC、IH的该抗原肌球蛋白2抗体5NM1 & 5NM2 检测原肌球蛋白。
研究子类别
肌肉生理学
肌肉生理学
研究类别
代谢
代谢
蛋白质印迹:1:100-1:500。 该抗体与29-30kDa的条带反应。
免疫细胞化学:1:100-1:500
免疫组化:1:100-1:500
最佳工作稀释度必须由最终用户确定。
免疫细胞化学:1:100-1:500
免疫组化:1:100-1:500
最佳工作稀释度必须由最终用户确定。
外形
溶于PBS的亲和纯化免疫球蛋白(液体)。
储存及稳定性
自收到之日起,在 -20°C 以未稀释的等分试样可保存 6 个月。应避免反复冻/融循环。
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
The Journal of biological chemistry, 289(17), 11616-11629 (2014-03-20)
Tropomodulins (Tmods) are F-actin pointed end capping proteins that interact with tropomyosins (TMs) and cap TM-coated filaments with higher affinity than TM-free filaments. Here, we tested whether differences in recognition of TM or actin isoforms by Tmod1 and Tmod3 contribute
Molecular biology of the cell, 26(9), 1699-1710 (2015-02-27)
Short, uniform-length actin filaments function as structural nodes in the spectrin-actin membrane skeleton to optimize the biomechanical properties of red blood cells (RBCs). Despite the widespread assumption that RBC actin filaments are not dynamic (i.e., do not exchange subunits with
The Journal of cell biology, 194(1), 105-120 (2011-07-06)
The sarcoplasmic reticulum (SR) serves as the Ca(2+) reservoir for muscle contraction. Tropomodulins (Tmods) cap filamentous actin (F-actin) pointed ends, bind tropomyosins (Tms), and regulate F-actin organization. In this paper, we use a genetic targeting approach to examine the effect
Molecular biology of the cell, 27(19), 2889-2897 (2016-08-19)
Tropomyosin (Tpm) isoforms decorate actin with distinct spatial and temporal localization patterns in cells and thus may function to sort actomyosin processes by modifying the actin track affinity for specific myosin isoforms. We examined the effect of three Tpm isoforms
The Journal of biological chemistry, 293(3), 863-875 (2017-12-02)
The metazoan actin cytoskeleton supports a wide range of contractile and transport processes. Recent studies have shown how the dynamic association with specific tropomyosin isoforms generates actin filament populations with distinct functional properties. However, critical details of the associated molecular
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