Anti-Cocaine and Amphetamine Related Transcript Antibody

Recognizes Cocaine- and Amphetamine Related Transcript (CART). CART has been found to control satiety modulating the actions of two key regulators of food intake, leptin and NPY. Starvation decreases CART levels in the arcuate nucleus (Kristensen et al. 1998). Obese animals have virtually no CART. CART inhibits both normal and starvation-induced feeding, and completely inhibits NPY-induced feeding. Immunoneutralization of CART by administration of anti-CART resulted into higher food intake suggesting that CART is an endogenous regulator of food intake.

Mouse/rat CART peptide (55-102).

Research Category
Neuroscience

Research Sub Category
CNS Control of Metabolism

Reward & Addiction

This Anti-Cocaine & Amphetamine Related Transcript Antibody is validated for use in ELISA, WB, IH for the detection of Cocaine & Amphetamine Related Transcript.

Western blotting: 1-10 μg/mL using ECL.

Immunohistochemistry: 5-10 μg/mL It has been reported that the antibody will recognize CART in neurons (cell bodies) from the rat hypothalamic arcuate nucleus.

ELISA: 1:10,000-1:50,000 using 50-100 ng control peptide (Catalog Number AG228) per well.

Optimal working dilutions must be determined by the end user.

Affinity purified immunoglobulin. Liquid in PBS containing 0.1% BSA. No preservative.

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.