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所有图片(1)

文件

AB5302

Sigma-Aldrich

淀粉样前体蛋白抗体,KPI结构域

serum, Chemicon®

别名:

APP

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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物来源

rabbit

质量水平

抗体形式

serum

抗体产品类型

primary antibodies

克隆

polyclonal

种属反应性

monkey, mouse, human, bovine

制造商/商品名称

Chemicon®

技术

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

UniProt登记号

运输

dry ice

靶向翻译后修饰

unmodified

基因信息

human ... APP(351)

相关类别

特异性

淀粉样前体蛋白(APP),KPI结构域。

免疫原

APP。

应用

淀粉样前体蛋白抗体(KPI结构域)是一种针对淀粉样前体蛋白的抗体,可用于IP、WB、IC、IH。
蛋白质印迹:1:250 - 1:1,000

免疫沉淀:需要对二硫键进行还原和烷基化。

免疫细胞化学:对于Cos7和NteraZ细胞系,需要对二硫键进行还原和烷基化。

免疫组化:用于甲醛固定、石蜡包埋的组织时,需要对二硫键进行还原和烷基化或微波。

最佳工作稀释度必须由最终用户进行确定。

还原/烷基化方案:

在黑暗中将载玻片(印迹)在TBS(PBS)+ 50mM 2-巯基乙醇中37°C孵育3小时进行还原。用TBS洗涤两次,然后在TBS(PBS)+ 20 mg/ml碘乙酸中室温孵育30 min。用TBS(PBS)洗涤两次,然后正常封闭和处理。

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Smith P, Al Hashimi A, Girard J, Delay C, Hebert SS.
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Early embryonic endocardium undergoes endothelial-to-mesenchymal transition to form cardiac cushion mesenchymal cells (MCs). Embryonic endocardium also gives rise to fibroblasts, intramyocardial adipocytes, and coronary mural cells, including smooth muscle cells and pericytes, in development. Whether endocardial cells directly differentiate into

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