生物来源
rabbit
质量水平
抗体形式
serum
抗体产品类型
primary antibodies
克隆
polyclonal
种属反应性
monkey, mouse, human, bovine
制造商/商品名称
Chemicon®
技术
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
UniProt登记号
运输
dry ice
靶向翻译后修饰
unmodified
基因信息
human ... APP(351)
特异性
淀粉样前体蛋白(APP),KPI结构域。
免疫原
APP。
应用
淀粉样前体蛋白抗体(KPI结构域)是一种针对淀粉样前体蛋白的抗体,可用于IP、WB、IC、IH。
蛋白质印迹:1:250 - 1:1,000
免疫沉淀:需要对二硫键进行还原和烷基化。
免疫细胞化学:对于Cos7和NteraZ细胞系,需要对二硫键进行还原和烷基化。
免疫组化:用于甲醛固定、石蜡包埋的组织时,需要对二硫键进行还原和烷基化或微波。
最佳工作稀释度必须由最终用户进行确定。
还原/烷基化方案:
在黑暗中将载玻片(印迹)在TBS(PBS)+ 50mM 2-巯基乙醇中37°C孵育3小时进行还原。用TBS洗涤两次,然后在TBS(PBS)+ 20 mg/ml碘乙酸中室温孵育30 min。用TBS(PBS)洗涤两次,然后正常封闭和处理。
免疫沉淀:需要对二硫键进行还原和烷基化。
免疫细胞化学:对于Cos7和NteraZ细胞系,需要对二硫键进行还原和烷基化。
免疫组化:用于甲醛固定、石蜡包埋的组织时,需要对二硫键进行还原和烷基化或微波。
最佳工作稀释度必须由最终用户进行确定。
还原/烷基化方案:
在黑暗中将载玻片(印迹)在TBS(PBS)+ 50mM 2-巯基乙醇中37°C孵育3小时进行还原。用TBS洗涤两次,然后在TBS(PBS)+ 20 mg/ml碘乙酸中室温孵育30 min。用TBS(PBS)洗涤两次,然后正常封闭和处理。
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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