产品名称
抗囊泡GABA转运蛋白(VGAT)抗体, Chemicon®, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, monkey, human, rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... SLC32A1(140679)
Analysis Note
对照
脑组织、大鼠视网膜裂解物
脑组织、大鼠视网膜裂解物
已通过蛋白质印迹对大鼠视网膜裂解物进行了评估。
蛋白质印迹分析:
该抗体的1:500的稀释液在10 μg大鼠视网膜裂解物中检测到囊泡GABA转运蛋白。
蛋白质印迹分析:
该抗体的1:500的稀释液在10 μg大鼠视网膜裂解物中检测到囊泡GABA转运蛋白。
Application
免疫组化/免疫荧光
在多聚甲醛固定切片上使用2-20 µg/mL的先前批次。
ELISA:
将先前批次的1:10,000–1:100,000稀释液用于ELISA,每孔使用50-100 ng对照肽(目录号AG379)。
蛋白质印迹:
1-10 µg/ml(化学发光技术)预期的分子量约为55-60 kDa。
最佳工作稀释度必须由最终用户进行确定。
在多聚甲醛固定切片上使用2-20 µg/mL的先前批次。
ELISA:
将先前批次的1:10,000–1:100,000稀释液用于ELISA,每孔使用50-100 ng对照肽(目录号AG379)。
蛋白质印迹:
1-10 µg/ml(化学发光技术)预期的分子量约为55-60 kDa。
最佳工作稀释度必须由最终用户进行确定。
该抗囊泡GABA转运蛋白(VGAT)抗体经验证可用于ELISA、IF、IH、WB检测囊泡GABA转运蛋白(VGAT)。
Biochem/physiol Actions
对囊泡GABA转运蛋白(VGAT)具有特异性。囊泡GABA转运蛋白(unc-47)同源物是525个氨基酸长的蛋白,属于神经递质转运蛋白家族。 免疫原肽与其他已知蛋白质没有显著同源性。
抗体与各种物种的交叉反应性尚不清楚免疫原序列在小鼠、人和猴中100%保守。
General description
57kda
囊泡GABA转运蛋白(unc-47)同源物是525个氨基酸长的蛋白,属于神经递质转运蛋白家族(1)。 VGAT在将GABA和甘氨酸摄取到突触囊泡中起作用。 由于蛋白与突触囊泡结合,因此VGAT抗体是突触前GABA能和甘氨酸能神经元的极佳标记。
Immunogen
大鼠VGAT羧基末端区域附近的17个氨基酸肽序列(McIntire,S.,1997)。
Other Notes
浓度:请参考批次特异性浓缩物的分析证书。
Physical form
在含有0.1%BSA的PBS缓冲液中的纯化兔多克隆抗体。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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