产品名称
抗谷胱甘肽抗体,可检测GSH & GSSG, Chemicon®, from rabbit
biological source
rabbit
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
eukaryotes
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... GSS(2937)
Application
蛋白质印迹(Mawatari和Murakami,2004)。
最佳工作稀释度必须由最终用户进行确定。
注意:谷胱甘肽不耐受锇,因此不应用于包埋后电子显微镜免疫组织化学。
所有程序均可在室温下进行。虽然不能预测所有应用的精确稀释度,但不太可能需要偏离校准浓度。100倍稀释液经校准,以优化2 log单位范围内的抗原水平检测。如果预期细胞内半抗原水平异常升高(>20 mM)或降低(<0.2 mM),或存在新的标本条件,如低戊二醛水平或不寻常的包埋方案,可以采用更高或更低的稀释比。更大的稀释比可能导致最大信号强度降低,而更浓的溶液将压缩检测的动态范围。
技术笔记
AB5010针对免疫金或荧光检测进行了优化。可以使用酶联法进行可视化,但通常会压缩检测的动态范围。使用高增益方法,如酪酰胺,则需要用户进行独立校准。冷冻切片是可行的,但不能产生最佳图像,因为IgG很难穿透乙醛交联组织,而且大多数氨基酸的含量过高,会产生丙氨酸效应。鉴于类似原因,不建议在整个安装座或振动切片机部分使用。
氧化应激
神经科学
Biochem/physiol Actions
Disclaimer
Immunogen
Packaging
Physical form
Preparation Note
Legal Information
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