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Merck
CN

AB5010

抗谷胱甘肽抗体,可检测GSH & GSSG

Chemicon®, from rabbit

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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
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产品名称

抗谷胱甘肽抗体,可检测GSH & GSSG, Chemicon®, from rabbit

biological source

rabbit

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

eukaryotes

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

Gene Information

human ... GSS(2937)

Application

免疫组化使用银强化免疫金或增强荧光法。当使用戊二醛固定剂时,通常需要用自动荧光还原剂处理。样品应使用0.2%-2.5%戊二醛固定,以实现最佳检测。参见技术笔记。 该抗体也已使用,并发现对零-低戊二醛/高多聚甲醛固定起作用(含4%多聚甲醛的0.1 M磷酸盐缓冲液/3%蔗糖固定剂)。AB5010的最小戊二醛浓度为0.02%。当使用渗透试剂(例如0.3%Triton X-100)时,冷冻切片,振动切片和组织培养形式的性能良好(仅在阻断缓冲液中,抗体稀释缓冲液应仅在简单TBS或PBS中)。

蛋白质印迹(Mawatari和Murakami,2004)。

最佳工作稀释度必须由最终用户进行确定。

注意:谷胱甘肽不耐受锇,因此不应用于包埋后电子显微镜免疫组织化学。



所有程序均可在室温下进行。虽然不能预测所有应用的精确稀释度,但不太可能需要偏离校准浓度。100倍稀释液经校准,以优化2 log单位范围内的抗原水平检测。如果预期细胞内半抗原水平异常升高(>20 mM)或降低(<0.2 mM),或存在新的标本条件,如低戊二醛水平或不寻常的包埋方案,可以采用更高或更低的稀释比。更大的稀释比可能导致最大信号强度降低,而更浓的溶液将压缩检测的动态范围。



技术笔记

AB5010针对免疫金或荧光检测进行了优化。可以使用酶联法进行可视化,但通常会压缩检测的动态范围。使用高增益方法,如酪酰胺,则需要用户进行独立校准。冷冻切片是可行的,但不能产生最佳图像,因为IgG很难穿透乙醛交联组织,而且大多数氨基酸的含量过高,会产生丙氨酸效应。鉴于类似原因,不建议在整个安装座或振动切片机部分使用。
研究子类别
氧化应激
研究类别
神经科学
该抗谷胱甘肽抗体可检测GSH & GSSG,经验证可用于IH中谷胱甘肽的检测。

Biochem/physiol Actions

谷胱甘肽。该抗体已针对一系列抗原进行了校准,以确保半抗原的选择性和适当的亲和力。对L-丙氨酸、γ-氨基丁酸、1-氨基-4-胍基丁烷(AGB)、D/L-精氨酸、D/L-门冬氨酸、L-瓜氨酸、L-半胱氨酸、D/L-谷氨酸、D/L-谷氨酰胺、谷胱甘肽、L-赖氨酸、L-鸟氨酸、L-丝氨酸、甘氨酸、L-苏氨酸、L-色氨酸、L-酪氨酸没有可测量的戊二醛固定组织交叉反应性(<1:1000)。 不区分游离GSH和GSSG。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

Immunogen

表位:检测GSH & GSSG
谷胱甘肽-戊二醛

Packaging

足够进行2000次测定

Physical form

形式:纯化
无菌0.1 M磷酸盐缓冲液中的IgG组分。不含防腐剂

Preparation Note

库存以未稀释的等分试样可在2-8°C保存长达6个月。该库存在正常使用和2-8°C常规储存下非常稳定。不要重复冷冻该库存。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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