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主要文件

AB3849-I

Sigma-Aldrich

抗磷酸SMAD2抗体（Ser465/467）

from rabbit, purified by affinity chromatography

别名:

Mothers against decapentaplegic homolog 2, Ser465/467 phosphorylated, hMAD-2, Ser465/467 phosphorylated, hSMAD2, Ser465/467 phosphorylated, JV18-1, Ser465/467 phosphorylated, MAD homolog 2, Ser465/467 phosphorylated, Mad-related protein 2, Ser465/467 pho

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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

CN¥2,240.04

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生物来源

rabbit

质量水平

100

抗体形式

affinity isolated antibody

抗体产品类型

primary antibodies

克隆

polyclonal

纯化方式

affinity chromatography

种属反应性

human

种属反应性（根据同源性预测）

zebrafish (based on 100% sequence homology), Xenopus (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology), chicken (based on 100% sequence homology), rat (based on 100% sequence homology)

技术

western blot: suitable

NCBI登记号

NP_005892

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HNDG4MAG36K

MILLIPLEX® Human Neurodegenerative Disease Magnetic Bead Panel 4 - Neuroscience Multiplex Assay

MILLIPLEX® Human Neuroscience Magnetic Bead Panel 1 - Neuroscience Multiplex Assay allows quantitative multiplex detection of multiple analytes simultaneously

HNS1MAG95K

MILLIPLEX® Human Neuroscience Magnetic Bead Panel 1 - Neuroscience Multiplex Assay

MILLIPLEX® Human Neurological Disorders Magnetic Bead Panel 3 - Neuroscience Multiplex Assay The analytes available for this multiplex kit are: AGT, Contactin-1, Fetuin A, Kallikrein-6, OPN, sSOD1, sSOD2

HND3MAG39K

MILLIPLEX® Human Neurological Disorders Magnetic Bead Panel 3 - Neuroscience Multiplex Assay

MILLIPLEX® Human Neurodegenerative Disease Magnetic Bead Panel 3 - Neuroscience Multiplex Assay The analytes available for this multiplex kit are: BDNF, Cathepsin D, MPO, NCAM, PAI-1 (total), PDGF-AA, PDGF-AB/BB, RANTES, sICAM-1, sVCAM-1.

HNDG3MAG36K

MILLIPLEX® Human Neurodegenerative Disease Magnetic Bead Panel 3 - Neuroscience Multiplex Assay

assay range

accuracy: 84-108%, standard curve range: 21-15,000 pg/mL
(sRAGE), standard curve range: 3-2,500 pg/mL
(Amyloid β40), standard curve range: 27-20,000 pg/mL
(Amyloid β42), standard curve range: 14-10,000 pg/mL
(S100B), standard curve range: 7-5,000 pg/mL
(GDNF)

assay range

accuracy: 107%
(DJ1), accuracy: 108%
(a-Synuclein), accuracy: 123%
(TG2), standard curve range: 12-50,000 pg/mL
(GFAP), standard curve range: 15-60,000 pg/mL
(NSE), standard curve range: 20-80,000 pg/mL
(UCHL1), standard curve range: 244-1,000,000 pg/mL
(alpha-synuclein), standard curve range: 61-250,000 pg/mL
(DJ1), standard curve range: 61-250,000 pg/mL
(TG2)

assay range

accuracy: 69-92%
(Cerebrospinal fluid (CSF)), accuracy: 71-107%
(Serum), standard curve range: 0.03-25 ng/mL
(SOD2), standard curve range: 0.04-30 ng/mL
(Kallikrein-6), standard curve range: 0.04-30 ng/mL
(SOD1), standard curve range: 0.08-60 ng/mL
(Contactin-1), standard curve range: 0.14-100 ng/mL
(Osteopontin (OPN)), standard curve range: 1.37-1,000 ng/mL
(AGT), standard curve range: 4.12-3,000 ng/mL
(Fetuin A)

assay range

accuracy: 108%
(RANTES), accuracy: 108%
(sICAM-1), accuracy: 111%
(Cathepsin D), accuracy: 123%
(PDGF-AA), accuracy: 137%
(PDGF-AB/BB), standard curve range: 2-10,000 pg/mL
(RANTES), standard curve range: 2.4-10,000 pg/mL
(BDNF), standard curve range: 2.4-10,000 pg/mL
(PAI-1 (total)), standard curve range: 2.4-10,000 pg/mL
(PDGF-AA), standard curve range: 24-100,000 pg/mL
(Cathepsin D), standard curve range: 24-100,000 pg/mL
(MPO), standard curve range: 24-100,000 pg/mL
(NCAM), standard curve range: 24-100,000 pg/mL
(PDGF-AB/BB), standard curve range: 24-100,000 pg/mL
(sICAM-1), standard curve range: 61-250,000 pg/mL
(sVCAM-1)

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

一般描述

母亲抗decapentaplegic同源物2（UniProt Q15796；也称为hMAD-2，hSMAD2，JV18-1，MAD同源物2，Mad相关蛋白2，母亲抗DPP同源物2，SMAD 2，SMAD家族成员2，Smad2）由人的SMAD2（也称为MADH2，MADR2）基因（基因ID 4087）编码。Smad2是通过转化生长因子TGF-β和激活素1型受体激酶激活的受体调节的细胞内信号转换器和转录调节剂。响应于TGF-β信号，smad2通过其与受体激活（SARA）蛋白的SMAD锚的相互作用而被募集到活化的TGF-β受体，其中它被TGF-β受体磷酸化。磷酸化导致其从SARA分离，并与另一个家族成员smad4结合。然后，smad2-smad4复合物易位到细胞核中，在细胞核中它与靶启动子结合并与其他辅因子形成转录阻遏物复合物。Smad2也可以被激活素1型受体激酶磷酸化，从而转导激活素依赖性信号传导事件。

特异性

该抗体可识别TGFβ刺激的HepG2细胞（而非未刺激的HepG2细胞）中的Smad2 Ser465/467磷酸化。选择性剪接的人Smad2Deltaexon3（短亚型）中相应的目标磷酸丝氨酸残基是pSer435 &pSer437（UniProt Q15796-2）。

免疫原

KLH偶联的合成磷酸肽，对应于人Smad2的Ser465/467周围的残基。
表位：磷酸-Ser465/467。

应用

抗磷酸SMAD2抗体（Ser465/467）是用于蛋白质印迹的针对磷酸SMAD2的抗体。
研究子类别
转录因子
研究类别
表观遗传学&核功能

质量

通过蛋白质印迹对HepG2细胞裂解液进行了评估。

蛋白质印迹分析：2.0 µg/mL该抗体在10 µg经TGFβ刺激而非未刺激的HepG2细胞裂解物中检测到Smad2 Ser465/467磷酸化。

目标描述

计算值~53kDa 52.31 kDa（长亚型）和48.96 kDa（短亚型；Smad2Deltaexon3）。

联系

替代品：AB3849

外形

亲和纯化
纯化的兔多克隆抗体，溶于含有0.1 M Tris-甘氨酸（pH 7.4）、150 mM NaCl和0.05％叠氮化钠的缓冲液中。

储存及稳定性

自收到之日起，在 2-8°C 条件下可稳定保存1年

其他说明

浓度：请参考特定批次的数据表。

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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