AB3849
Anti-Smad2 Antibody, phospho-specific (Ser465/467)
别名:
MAD, Mad, Mad protein homolog 2, Mad-related protein 2, Mothers against DPP homolog 2, SMAD, SMAD 2, SMAD family member 2, Sma- and Mad-related protein 2, mother against DPP homolog 2, mothers against DPP homolog 2, mothers against DPP homolog 2 (Drosophila), mothers against decapentaplegic homolog 2, mothers against decapentaplegic homolog 2 (Drosophila)
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About This Item
UNSPSC代码:
12352203
eCl@ss:
32160702
推荐产品
生物来源
rabbit
抗体形式
affinity purified immunoglobulin
克隆
polyclonal
纯化方式
affinity chromatography
using Protein A
种属反应性
zebrafish (100% sequence similarity), mouse, human, rat, Xenopus (100% sequence similarity), chicken (100% sequence similarity)
制造商/商品名称
Chemicon®
技术
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI登记号
UniProt登记号
一般描述
SMAD2 or Mothers against decapentaplegic homolog 2 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decepentaplegic". It belongs to the SMAD family of proteins, which belong to the TGF-β superfamily of modulators. Like many other TGF-β family members SMAD2 is involved in cell signaling. SMAD2 modulates signals of activin and TGF-β′s. It interacts with SMAD anchor for receptor activation (SARA). The binding of ligands causes the phosphorylation of the SMAD2 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD2 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.
特异性
By western blot, the antibody recognizes a band migrating at approximately 58 kDa corresponding to Smad2 only when dually phosphorylated at Ser465 and Ser467. The antibody may also detect phosphorylated Smad3 (Ser423 and Ser425). This antibody does not c
免疫原
Epitope: Phospho-Ser465/467
KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Ser465/467 of human Smad2.
应用
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Use Anti-Smad2 Antibody, phospho-specific (Ser465/467) (Rabbit Polyclonal Antibody) validated in ICC, IF, IHC(P), WB to detect Smad2 also known as MAD-mothers against decapentaplegic-homolog 2.
Western Blotting:
1:1,000 dilution of a previous lot was used. For best results, incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C overnight with gentle agitation.
Immunohistochemistry (Paraffin):
1:2000 dilution of a previous lot was used.
Immunocytochemistry (IF):
1:500 dilution of a previous lot was used.
Optimal working dilutions must be determined by the end user.
1:1,000 dilution of a previous lot was used. For best results, incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C overnight with gentle agitation.
Immunohistochemistry (Paraffin):
1:2000 dilution of a previous lot was used.
Immunocytochemistry (IF):
1:500 dilution of a previous lot was used.
Optimal working dilutions must be determined by the end user.
目标描述
~58 kDa
外形
Purified rabbit polyclonal in buffer containing 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA, with 50% glycerol.
储存及稳定性
Stable for 1 year at -20ºC from date of receipt.
分析说明
Control
POSITIVE CONTROL: TGF-β treated HepG2 cells.
POSITIVE CONTROL: TGF-β treated HepG2 cells.
其他说明
Concentration:Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
法规信息
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