Anti-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody, phospho-specific

Recognizes Thr567 phosphorylated ezrin, Thr564 phosphorylated radixin, and Thr558 phosphorylated moesin. Antibody does not cross-react with other related phospho proteins such as merlin or band 4.1.

Epitope: phospho-specific

KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Thr567 of human ezrin

Anti-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody, phospho-specific detects level of Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) & has been published & validated for use in IH(P) & WB.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Western blotting 1:1,000 (for best results, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight). The antibody recognizes 75KDa (Moesin, and ~80kDa Ezrln & Radixin) bands.

Immunohistochemistry (paraffin): 1:50

Optimal working dilutions must be determined by the end user.

75KDa Moesin, and ~80kDa Ezrin & Radixin

ImmunoAffinity Purified

Liquid in 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 μg/ml BSA, with 50% glycerol.

Maintain at -20°C for 12 months, do not aliquot.

Control
Cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.