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生物来源
rabbit
抗体形式
affinity purified immunoglobulin
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性（根据同源性预测）
all
制造商/商品名称
Chemicon®
技术
immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
运输
wet ice
靶向翻译后修饰
unmodified
特异性
T7 (MASMTGGQQMG). Rabbits were immunized with T7 (MASMTGGQQMG) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
免疫原
Epitope: T7 (MASMTGGQQMG)
MASMTGGQQMG (T7) conjugated to KLH
应用
Anti-T7 Epitope Tag Antibody, T7 (MASMTGGQQMG) detects level of T7 Epitope Tag & has been published & validated for use in IP, WB & IC.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Epitope Tags
Western blot: 1:1,000-1:10,000 colorimetric; 1:1,000-1:30,000 ECL.
Immunocytochemistry: 1:100-1:400
ELISA: 1:100-1:500 for coating; 1:1,000-1:30,000 for primary.Immunofluorescence-suitable
Immunoprecipitation: 2-5μg/mL in 300-500μL of cell lysate (150-300μg/mL total protein). Lysis buffer 0.5% triton X-100 with proteinase inhibitors. Use high quality protein A beads or anti-rabbit conjugated beads for best results. Run bead-only (no T7 antibody) controls to mark bands related to protein A breakdown products.
Optimal working dilutions must be determined by the end user.
Immunocytochemistry: 1:100-1:400
ELISA: 1:100-1:500 for coating; 1:1,000-1:30,000 for primary.Immunofluorescence-suitable
Immunoprecipitation: 2-5μg/mL in 300-500μL of cell lysate (150-300μg/mL total protein). Lysis buffer 0.5% triton X-100 with proteinase inhibitors. Use high quality protein A beads or anti-rabbit conjugated beads for best results. Run bead-only (no T7 antibody) controls to mark bands related to protein A breakdown products.
Optimal working dilutions must be determined by the end user.
外形
Affinity purified immunoglobulin. Liquid in PBS, pH 7.2 with 0.1% sodium azide.
储存及稳定性
Maintain at 2-8°C in undiluted for up to 6 months from date of receipt.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Lucas J McKinnon et al.
The Plant cell, 32(5), 1589-1609 (2020-03-15)
Protein folding is a complex cellular process often assisted by chaperones, but it can also be facilitated by interactions with lipids. Disulfide bond formation is a common mechanism to stabilize a protein. This can help maintain functionality amid changes in
Jayanth Kumar Palanichamy et al.
The Journal of clinical investigation, 126(4), 1495-1511 (2016-03-15)
Posttranscriptional control of gene expression is important for defining both normal and pathological cellular phenotypes. In vitro, RNA-binding proteins (RBPs) have recently been shown to play important roles in posttranscriptional regulation; however, the contribution of RBPs to cell specification is
Blanca Chinchilla et al.
Fish & shellfish immunology, 70, 343-350 (2017-09-09)
Non-virion (NV) protein is essential for an efficient replication increasing the pathogenicity of the Salmonid novirhabdovirus (formerly IHNV), Piscine novirhabdovirus (formerly VHSV), and Hirame novirhabdovirus (HIRV). The interferon system, apoptosis, and other immune-related genes are modulated by NV to finally
Hyung-Joon Kwon et al.
Nature communications, 7, 11686-11686 (2016-05-26)
NF-κB is a key transcription factor that dictates the outcome of diverse immune responses. How NF-κB is regulated by multiple activating receptors that are engaged during natural killer (NK)-target cell contact remains undefined. Here we show that sole engagement of
Dae Kwan Ko et al.
Nature plants, 9(8), 1333-1346 (2023-08-11)
Excessive accumulation of misfolded proteins in the endoplasmic reticulum (ER) causes ER stress, which is an underlying cause of major crop losses and devastating human conditions. ER proteostasis surveillance is mediated by the conserved master regulator of the unfolded protein
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