产品名称
Anti-DEDAF Antibody, Chemicon®, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, rat, mouse
manufacturer/tradename
Chemicon®
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... RYBP(23429)
Application
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-DEDAF Antibody is validated for use in WB for the detection of DEDAF.
Western blot: 0.5-1 μg/mL.
A549 or HepG2 cell lysate can be used as a positive control and a 32 kDa band should be detected.
Optimal working dilutions must be determined by end user.
A549 or HepG2 cell lysate can be used as a positive control and a 32 kDa band should be detected.
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. DEDAF (DED Associated Factor) is expressed in multiple tissues and cell lines. DEDAF is identical to the transcriptional repressor RYBP. DEDAF interacts with FADD and augments the formation of CD95/FADD/Caspase-8 complexes at the cell membrane. DEDAF also interacts with DEDD (DED-containing DNA binding) protein in the nucleus, which indicates its involvement in the regulation of both cytoplasmic and nuclear events of apoptosis.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Rabbit anti-DEDAF polyclonal antibody was raised against a synthetic peptide corresponding to amino acids 215 to 228 of human-DEDAF. This sequence is identical to the mouse sequence.
Physical form
Format: Purified
Purified IgG by immunoaffinity chromatography. Liquid in PBS containing 0.02% sodium azide.
Preparation Note
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Izabella Bajusz et al.
Genes, 10(11) (2019-11-23)
Separation of germline cells from somatic lineages is one of the earliest decisions of embryogenesis. Genes expressed in germline cells include apoptotic and meiotic factors, which are not transcribed in the soma normally, but a number of testis-specific genes are
Enikő Sutus et al.
Scientific reports, 12(1), 2364-2364 (2022-02-13)
We have previously reported that RING1 and YY1 binding protein (RYBP) is important for central nervous system development in mice and that Rybp null mutant (Rybp-/-) mouse embryonic stem (ES) cells form more progenitors and less terminally differentiated neural cells
Paula Dobrinić et al.
Nature structural & molecular biology, 28(10), 811-824 (2021-10-06)
The Polycomb repressive system plays a fundamental role in controlling gene expression during mammalian development. To achieve this, Polycomb repressive complexes 1 and 2 (PRC1 and PRC2) bind target genes and use histone modification-dependent feedback mechanisms to form Polycomb chromatin
Rybp/DEDAF is required for early postimplantation and for central nervous system development.
Pirity, MK; Locker, J; Schreiber-Agus, N
Molecular and cellular biology null
Dynamic antagonism between key repressive pathways maintains the placental epigenome.
Weigert, et al.
Nature Cell Biology, 25, 579-591 (2023)
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