产品名称
Anti-Adiponectin Antibody, Chemicon®, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ADIPOQ(9370)
Other Notes
Replaces: MABS176
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Purified immunoglobulin in PBS, pH 7.4.
Application
Anti-Adiponectin Antibody detects level of Adiponectin & has been published & validated for use in ELISA & WB.
Research Category
Metabolism
Metabolism
Research Sub Category
Lipid Metabolism & Weight Regulation
Lipid Metabolism & Weight Regulation
Western blot: 1:5,000-1:10,000 using ECL. Reacts with the ~35 kDa adiponectin protein. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk. Suggested incubation time is 1 hour at room temperature.
ELISA, direct and indirect: 1:5,000-1:10,000.
Optimal working dilutions must be determined by the end user.
ELISA, direct and indirect: 1:5,000-1:10,000.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Recognizes mouse adiponectin. Recognizes both natural and recombinant protein.
SPECIES REACTIVITIES:
There is weak reactivity with rat. Reactivity with other species has not been confirmed.
SPECIES REACTIVITIES:
There is weak reactivity with rat. Reactivity with other species has not been confirmed.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Recombinant mouse adiponectin from E. coli.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Longhua Liu et al.
Cell cycle (Georgetown, Tex.), 15(15), 2033-2041 (2016-06-05)
Obesity and related metabolic disorders constitute one of the most pressing heath concerns worldwide. Increased adiposity is linked to autophagy upregulation in adipose tissues. However, it is unknown how autophagy is upregulated and contributes to aberrant adiposity. Here we show
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