Anti-Myostatin Antibody, Near CT

Myostatin, also known as Growth/differentiation factor 8, is a negative regulator of skeletal muscle growth encoded by the MSTN (also known as MSLHP and GDF8) gene in human. Myostatin is a member of the bone morphogenetic protein (BMP) family within the TGF-ß superfamily. Growth and differentation factor-associated serum protein-1 & 2 (GASP-1/2) and follistatin-related gene (FLRG) protein are reported to interact with myostatin and negatively regulate its activity (PMID 24019467, 17878677). Mutation in MSTN gene is known to cause muscle hypertrophy (MSLHP), a condition characterized by increased muscle bulk and strength.

Epitope: Near C-terminal

KLH-conjugated linear peptide corresponding to human Myostatin near the C-terminal.

Anti-Myostatin Antibody, Near CT is an antibody against Myostatin, Near CT for use in Western Blotting and Immunohistochemistry.

Immunohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected Myostatin in human cardiac myocytes tissue.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Evaluated by Western Blotting in human adult heart tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Myostatin in 10 µg of human adult heart tissue lysate.

~31 kDa observed. 30–32 kDa band assumed to be a glycosylated dimer of the carboxy terminus portion of the full length translational product (Artaza, J.N., et al. (2007). J. of Endocrinology. 194:63-76). This antibody also detected the 32-kDa band corresponding to the processed dimer (Artaza, J.N., et al. (2007). J. of Endocrinology. 146(8):3547-3557).

Affinity

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.