Anti-Uncoupling Protein 2 Antibody

Uncoupling Protein 2 (UCP2), human. Human UCP2 is a 309 amino acid mitochondrial uncoupling protein (Fleury et al. 1997; Gimeon et al. 1997). It is only 59% homologous with UCP1 found in brown adipose tissues. UCP2 has wide tissue distribution in tissues (brain, kidney, liver, brown adipose tissue, heart, and muscle). UCP2 may play a critical role in energy balance, body weight, and thermoregulation (Fleury et al. 1997; Gimeon et al. 1997; Flier & Lowell 1997).

SPECIES REACTIVITIES: The antibody has not yet been tested for cross-reactivity to other species. The sequence used for immunization shows 100% homology to mouse and rat UCP2 and 92% homology with canine, porcine and bovine.

13 aa peptide sequence near the 3rd and 4th transmembrane domain of human UCP2 (Fleury et al. 1997; Gimeon et al. 1997). The peptide has no significant homology with UCP1, UCP3 or UCP4 (Boss et al. 1997).

Detect Uncoupling Protein 2 using this Anti-Uncoupling Protein 2 Antibody validated for use in ELISA & WB.

Research Category
Metabolism

Research Sub Category
Ion & Transport Channels

Western Blotting: 1-10 μg/mL using Chemiluminescence technique.

Immunohistochemistry: Has not been tested. It is recommended that you use 2-20 μg/mL on formaldehyde fixed tissue.

ELISA: 1:10,000-1:100,000; using 50-100 ng UCP2 control peptide/well.

Optimal working dilutions must be determined by end user.

Affinity purified immunoglobulin. Liquid in 100mM Tris, pH 7.5 with 0.2% BSA as stabilizer and 0.05% sodium azide.

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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