推荐产品
生物来源
rabbit
质量水平
抗体形式
affinity purified immunoglobulin
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
mouse
制造商/商品名称
Chemicon®
技术
ELISA: suitable
western blot: suitable
NCBI登记号
UniProt登记号
运输
dry ice
靶向翻译后修饰
unmodified
基因信息
mouse ... Ucp1(22227)
特异性
Uncoupling Protein 1 (UCP1), mouse. Mouse/rat UCP1 is a 307 amino acid mitochondrial uncoupling protein (Bouillaud et al. 1986; Ridley et al. 1986; Kozak et al. 1988; Cassard et al. 1990; Miroux et al. 1993). It is only 59% homologous with UCP2 (Fleury et al. 1997). Human UCP1 is located on chromosome 4 (Bouillaud et al. 1986; Ridley et al. 1986; Kozak et al. 1988; Cassard et al. 1990; Miroux et al. 1993). UCP1 is primarily expressed in brown adipose tissues.
The antibody has not yet been tested for cross-reactivity to other species. The sequence used for immunization shows 100% homology to rat, 91% with golden hamster, 83% with rabbit and 75% with human and canine UCP1.
The antibody has not yet been tested for cross-reactivity to other species. The sequence used for immunization shows 100% homology to rat, 91% with golden hamster, 83% with rabbit and 75% with human and canine UCP1.
免疫原
A 12 aa peptide at the C-terminus of the mouse/rat UCP1 (Bouillaud et al. 1986; Ridley et al. 1986; Kozak et al. 1988; Cassard et al. 1990; Miroux et al. 1993).
应用
Detect Uncoupling Protein 1 using this Anti-Uncoupling Protein 1 Antibody validated for use in ELISA & WB.
Western Blotting: 1-10 μg/mL using Chemiluminescence technique. An antibody made to this epitope has detected a protein of ~32 kDa in brown adipose tissues (Feil & Rafael 1994).
ELISA: 1:10,000-1:100,000; using 50-100 ng UCP1 control peptide/well.
Optimal working dilutions must be determined by end user.
ELISA: 1:10,000-1:100,000; using 50-100 ng UCP1 control peptide/well.
Optimal working dilutions must be determined by end user.
外形
Affinity purified immunoglobulin. Liquid in PBS with less than 0.1% sodium azide.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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