Anti-Integrin α4 Antibody, cytoplasmic on CT

Integrin alpha 4. Specificity determined by immunoprecipitation from a detergent extract of 35S methionine-labeled lymphoid cells. No cross-reactivity to alpha 1, alpha 2, alpha 3, alpha 5, alpha 6 or alpha V. The antibody recognizes an epitope on the cytoplasmic domain of the protein, and it is thus intracellular.

Epitope: cytoplasmic domain, C-terminus

Synthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human alpha 4 integrin subunit.

Anti-Integrin α4 Antibody, cytoplasmic on C-terminus is an antibody against Integrin α4 for use in ELISA, IP, RIA, WB, IH.

ELISA/RIA: 1:1000.

Western blot: 1:1000, non-reduced conditions; blocking should be with 1-3% BSA. Recognizes a 140 kDa human alpha 4 band under non-reducing conditions.

Immunohistochemistry: 1:500

Immunoprecipitation: 5 μL of antibody is sufficient to precipitate alpha4 from 5x106 cells.

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Integrins

Replaces: 04-1131

Liquid rabbit antiserum with 0.05% sodium azide.

Maintain at -20ºC in undiluted aliquots for up to 12 months upon date of receipt. Avoid repeated freeze/thaw cycles.

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