Anti-Caspase 8 Antibody

Caspase-8 is a 55 kDa cytosolic protein that is synthesized as an inactive proenzyme. Activation of caspase-8 involves a two-step proteolysis: the cleavage of caspase-8 to generate a 43 kDa fragment and a 12 kDa which is further processed to 10 kDa, and then the p43 is then cleaved to yield p26 and the release of the active site containing p18.

AB1879 detects 55 and 43 kDa proteins, corresponding to the proform and large subunit of caspase-8. The antibody also detects human recombinant caspase-8 protein on SDS-PAGE immunoblots.

recombinant, full length, purified human caspase 8

Research Category
Apoptosis & Cancer

Metabolism

Research Sub Category
Caspases

Enzymes & Biochemistry

This Anti-Caspase 8 Antibody is validated for use in WB for the detection of Caspase 8.

Western blot: 0.5 - 4 μg/mL

Optimal working dilutions must be determined by end user.

55 and 43 kDa

Replaces: 04-573; 04-574

ImmunoAffinity Purified

Liquid in PBS, pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thiomerosal. Affinity Purified immunoglobulin.

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Control
Skin/Tumor, Embryo

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.