生物来源
rabbit
质量水平
抗体形式
affinity purified immunoglobulin
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
human
制造商/商品名称
Chemicon®
技术
ELISA: suitable
neutralization: suitable
western blot: suitable
UniProt登记号
运输
dry ice
靶向翻译后修饰
unmodified
基因信息
human ... CXCL12(6387)
特异性
Reacts with human SCDF-1alpha
免疫原
Highly pure (>98%) recombinant human SDF-1alpha.
应用
Detect Stromal Cell-Derived Factor-1α using this Anti-Stromal Cell-Derived Factor-1α Antibody validated for use in ELISA, WB, NEUT.
ELISA: 0.5 μg/mL this dilution will allow detection (in direct ELISA) of 0.2-0.4 ng/well when human SDF-1alpha is used to coat the plate.
Western blot: 0.1-0.2 μg/mL, this dilution should offer a detection limit of 1.5-3.0 ng/lane, under reducing or non-reducing conditions.
Optimal working dilutions must be determined by the end user.
Neutralization: To yield one-half maximal inhibition (ND50) of the biological activity of human SDF-1alpha (100 ng/mL), a concentration of 2.0-0.4 ng/well of recombinant human SDF-1alpha
Western blot: 0.1-0.2 μg/mL, this dilution should offer a detection limit of 1.5-3.0 ng/lane, under reducing or non-reducing conditions.
Optimal working dilutions must be determined by the end user.
Neutralization: To yield one-half maximal inhibition (ND50) of the biological activity of human SDF-1alpha (100 ng/mL), a concentration of 2.0-0.4 ng/well of recombinant human SDF-1alpha
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors
Cytokines & Cytokine Receptors
外形
Affinity purified immunoglobulin. Lyophilized from 0.5X PBS pH 7.2. Reconstitute with sterile distilled water.
储存及稳定性
The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile water to a concentration of =0.2 mg/ml the antibody is stable for at least six weeks at 2-4°C.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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