产品名称
Anti-Connexin 43 Antibody, Chemicon®, from rabbit
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, bovine, rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... GJA1(2697)
Application
ELISA: 1:10,000-100,000 using 50 - 100 ng Cx43 control peptide per well.
Immunocytochemistry: not tested. It is recommended that the antibody be tried at 2-20μg/mL in formaldehyde fixed (Beyer et al. 1985; Nicholson et al. 1985; John et al. 1991; Fishman et al. 1990).
Immunoblotting: 1-10μg/mL using Chemiluminescence technique.
Optimal working dilutions must be determined by end user.
Immunohistochemistry: A 1:50 dilution of this antibody detected Connexin 43 in sections from mouse heart tissue pretreated with Tris-EDTA buffer, pH 9.0. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection.
Immunocytochemistry: not tested. It is recommended that the antibody be tried at 2-20μg/mL in formaldehyde fixed (Beyer et al. 1985; Nicholson et al. 1985; John et al. 1991; Fishman et al. 1990).
Immunoblotting: 1-10μg/mL using Chemiluminescence technique.
Optimal working dilutions must be determined by end user.
Immunohistochemistry: A 1:50 dilution of this antibody detected Connexin 43 in sections from mouse heart tissue pretreated with Tris-EDTA buffer, pH 9.0. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
This Anti-Connexin 43 Antibody is validated for use in ELISA, IP, WB, IC for the detection of Connexin 43.
Biochem/physiol Actions
Mouse Cx43 immunogenic peptide sequence is specific for Cx43 and no significant homology is seen with other connexins. The mouse Cx43 peptide sequence shows 100% conserved with rat and bovine, and 84% with chicken and human (16/19 aa) Cx43 (Beyer et al. 1985; Nicholson et al. 1985; John et al. 1991; Fishman et al. 1990).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Mouse Connexin 43 is a 382 amino acid gap junction protein withmouse connexin
a predicted M.W. of ~43 kDa. It is prominently expressed in heart (see reviews: Kumar & Giula 1996; White et al. 1995; Evans 1994; Beyer et al. 1990).
a predicted M.W. of ~43 kDa. It is prominently expressed in heart (see reviews: Kumar & Giula 1996; White et al. 1995; Evans 1994; Beyer et al. 1990).
Immunogen
KLH-conjugated synthetic peptide
corresponding to amino acids 360-382 within
the C-terminus of mouse connexin 43.
corresponding to amino acids 360-382 within
the C-terminus of mouse connexin 43.
Physical form
Affinity-purified using peptide-Sepharose column chromatography and supplied in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl containing 0.05% sodium azide.
Preparation Note
Store at 2-8°C for 1 year from date of receipt.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Vanessa M Mahoney et al.
Scientific reports, 6, 26744-26744 (2016-06-01)
Studies have demonstrated non-myocytes, including fibroblasts, can electrically couple to myocytes in culture. However, evidence demonstrating current can passively spread across scar tissue in the intact heart remains elusive. We hypothesize electrotonic conduction occurs across non-myocyte gaps in the heart
Elisa Ferraro et al.
Scientific reports, 10(1), 15284-15284 (2020-09-19)
Acute myocardial ischaemia and reperfusion (I-R) are major causes of ventricular arrhythmias in patients with a history of coronary artery disease. Ursodeoxycholic acid (UDCA) has previously been shown to be antiarrhythmic in fetal hearts. This study was performed to investigate
Jean-Baptiste Guichard et al.
Cardiovascular research, 117(2), 462-471 (2020-01-25)
No studies have assessed the specific contributions of atrial fibrillation (AF)-related atrial vs. associated ventricular arrhythmia to remodelling. This study assessed the roles of atrial arrhythmia vs. high ventricular rate in AF-associated remodelling. Four primary dog-groups (12/group) were subjected to 3-week
J E Rash et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 21(6), 1983-2000 (2001-03-14)
The transmembrane connexin proteins of gap junctions link extracellularly to form channels for cell-to-cell exchange of ions and small molecules. Two primary hypotheses of gap junction coupling in the CNS are the following: (1) generalized coupling occurs between neurons and
Khai Le Quang et al.
Circulation. Arrhythmia and electrophysiology, 8(4), 921-932 (2015-06-14)
Integrin-linked kinase (ILK), a serine/threonine protein kinase, has roles in cell signaling and molecular scaffolding. ILK mutation/deletion causes cardiomyopathic phenotypes, but the functional and electrophysiological features have not been characterized. This study investigated the structural, functional, ion channel, and electrophysiological
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