Anti-Fas Antibody

Human FAS Ligand (FASL). The 13-mer human FASL sequence used for antibody production is 92% (12/13 AA) homologous with rat and mouse FASL. Based upon the sequence homology data, anti-human FASL will likely cross react with the mouse/rat FASL.

A 13 AA peptide sequence mapping near the C-terminus of human FAS ligand (Mita et al. 1994). This peptide is predicted to be extracellular. The peptide was synthesized with C-terminal Cysteine, coupled to KLH.

Anti-Fas Ligand Antibody detects level of Fas Ligand & has been published & validated for use in ELISA & WB.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blotting: 1:1,000-1:5,000 Rat, mouse and human FASL have 278, 279, and 281 AA residues, respectively (calculated MW 32kDa). Western blot of the oocyte has detected a specific 31kDa band (Hakuno et al. 1996). A 40 kDa FASL has been detected in mouse T cells (Hahne 1995). An alternate detecting reagent, mFAS-Fc (Suda & Nagata 1994) also detected a cell surface protein of about 40 kDa.

ELISA: 0.5-1μg/mL

Optimal working dilutions must be determined by the end user.

Peptide affinity purified immunoglobulin. Liquid in PBS, containing 0.1% BSA.

Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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