Anti-ICAD/DFF Antibody, NT (a.a. 2-21 of mouse ICAD)

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated Dnase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis (Liu et al. 1997). Mouse homologue of human DFF was identified as a Dnase inhibitor designated ICAD (Enari et al. 1998; Sakahira et al. 1998). Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei (Enari et al. 1998; Sakahira et al. 1998). Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which cause Dnase activation and DNA degradation, is the hallmark of apoptotic cell death (Wylile et al. 1998).

Epitope: N-terminus (a.a. 2-21 of mouse ICAD)

Polyclonal antibody raised against a peptide corresponding to amino acids 2-21 of mouse ICAD (Enari et al. 1998).

Anti-ICAD/DFF Antibody, N-terminus (a.a. 2-21 of mouse ICAD) is an antibody against ICAD/DFF for use in WB.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western blot: 1:500-1:1000

Mouse lung tissue lysate can be used as a positive control and a 45 kDa band should be detected.

Optimal working dilutions must be determined by end user.

Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide.

Format: Purified

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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