Anti-DR4 Antibody, CT

Apoptosis, or programmed cell death, occurs during normal cellular diffferentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel cell death receptor was recently identified by several groups independently and designated DR4 (for death receptor 4) (Pan et al. 1997). The ligand for this novel cell death receptor has been identified and termed TRAIL, which is a new member in TRF family. Therefore, DR4 is also called TRAIL receptor-1 (TRAIL-R1) (Schneider et al. 1997). DR4 is expresssed in most of human tissues including spleen, peripheral blood leukocytes, small intestine and thymus. Like TNFR1, Fas and DR3, DR4 mediates TRAIL induced apoptosis and NF-kB activation in many tissues and cells.

Epitope: C-terminus

Polyclonal antibody raised against a peptide corresponding to amino acids 427-445 at C-terminus of human DR4 mature protein.

Detect DR4 using this Anti-DR4 Antibody, C-terminus validated for use in WB.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western blot: 1:500-1:1000

Whole cell lysate from HeLa cells can be used as a positive control and a 57 kDa band should be detected.

Optimal working dilutions must be determined by end user.

Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide.

Format: Purified

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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