Anti-Nerve Growth Factor-β Antibody, pain

Nerve growth factor-beta (NGF). Less than 1% cross-reactivity against recombinant human BDNF, NT3 and NT4 by ELISA.

Mouse nerve growth factor-beta purified from salivary glands.

Immunohistochemistry: 1-2 μg/mL (see suggested protocol).

Immunoblotting: 1-2 μg/mL

Bioassays (Inhibition of biological activity in vitro): 1-2 μg/mL

in vivo animal studies: use neat

ELISA: 1-2 μg/mL

Optimal working dilutions must be determined by the end user.

BIOLOGICAL ACTIVITY:

This antibody completely inhibits neuron survival and the outgrowth actions of mouse NGF in chicken dorsal root ganglion in vitro.

Research Category
Neuroscience

Research Sub Category
Neurochemistry & Neurotrophins

This Anti-Nerve Growth Factor-β Antibody, pain is validated for use in ELISA, WB, IH, FUNC for the detection of Nerve Growth Factor-β.

Replaces: 04-1119

Affinity purified immunoglobulin. Lyophilized from PBS. Contains no preservative. Reconstitute to desired volume with sterile distilled water. Suggested concentration 0.5-1.0 mg/mL.

Maintain at lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20 to -70°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.