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AB1252

Sigma-Aldrich

抗细胞色素P450 酶CYP2E1抗体

serum, Chemicon®

别名:

cytochrome P450 2E1, cytochrome P450, family 2, subfamily E, polypeptide 1, cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1, flavoprotein-linked monooxygenase, microsomal monooxygenase, xenobiotic monooxygenase

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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物来源

rabbit

质量水平

抗体形式

serum

抗体产品类型

primary antibodies

克隆

polyclonal

种属反应性

rat, human, mouse

制造商/商品名称

Chemicon®

技术

immunohistochemistry: suitable
western blot: suitable

NCBI登记号

UniProt登记号

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

human ... CYP2E1(1571)
mouse ... Cyp2E1(13106)
rat ... Cyp2E1(25086)

相关类别

一般描述

细胞色素P450 (CYP)是一个巨大的、多样化的血红素蛋白超家族。它可以将脂肪酸花生四烯酸(AA)代谢为血管紧张度的调节因子。
大多数CYP可以代谢多种底物,并且可以催化多种反应。这种作用导致它们在诸多内源性和外源性分子的代谢中极为重要。在肝脏中,这些底物包括药物和毒性化合物,以及代谢产物,如胆红素(血红蛋白的分解产物)。细胞色素P450 酶存在于机体的许多其它组织中,包括胃肠道的粘膜,并在激素合成和分解(包括雌激素和睾丸素合成和代谢)、胆固醇合成和维生素D代谢中起重要作用。自2007年9月以来,我们已经知道了7700多种不同的CYP序列。

特异性

与肝脏微粒体部分(microsomal fraction)中的人和大鼠细胞色素P450 酶CYP2E1反应。与这些物种中的其它细胞色素P450 酶没有交叉反应。

应用

免疫组化:
一个先前批次被用于甲醛处理的冷冻切片。

最佳的工作稀释度必须由最终用户确定。
抗细胞色素P450 酶CYP2E1抗体是针对细胞色素P450 酶CYP2E1的抗体,可用于IH & WB。

质量

通过蛋白质印迹对人脑裂解物进行了评估。

蛋白质印迹分析:
该抗体的1:1000稀释液在10 μg小人脑裂解物中检测到细胞色素P450 酶CYP2E1。

目标描述

57kda

外形

不含防腐剂的缓冲液中的兔多克隆抗血清。

分析说明

对照
肝脏组织。

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Kyle J Thompson et al.
Alcohol and alcoholism (Oxford, Oxfordshire), 52(6), 629-637 (2017-10-17)
This study sought to compare mice bred to preferentially consume high amounts of alcohol (crossed-high alcohol preferring, cHAP) to c57BL/6 (C57) mice using a chronic-binge ethanol ingestion model to induce alcoholic liver disease (ALD). Male C57 and cHAP mice were
Pengcheng Wang et al.
Biochemical pharmacology, 145, 218-225 (2017-09-11)
Acetylation is the major metabolic pathway of isoniazid (INH) mediated by N-acetyltransferases (NATs). Previous reports suggest that slow acetylators have higher risks of INH hepatotoxicity than rapid acetylators, but the detailed mechanisms remain elusive. The current study used Nat1/2(-/-) mice
Rebecca L McCullough et al.
Molecular immunology, 75, 122-132 (2016-06-10)
Complement is implicated in the development of alcoholic liver disease. C3 and C5 contribute to ethanol-induced liver injury; however, the role of C5a receptor (C5aR) on myeloid and non-myeloid cells to progression of injury is not known. C57BL/6 (WT), global
Thomas Leibing et al.
Hepatology (Baltimore, Md.), 68(2), 707-722 (2017-10-24)
Postnatal liver development is characterized by hepatocyte growth, proliferation, and functional maturation. Notably, canonical Wnt signaling in hepatocytes has been identified as an important regulator of final adult liver size and metabolic liver zonation. The cellular origin of Wnt ligands
Xiaoxia Jin et al.
Frontiers in pharmacology, 9, 1317-1317 (2018-12-14)
This study was designed to explore the role of cytochrome P4502E1 (CYP2E1) expression in the course of brain edema induced by subacute poisoning with 1,2-dichloroethane (1,2-DCE). Mice were randomly divided into five groups: the control group, the 1,2-DCE poisoned group

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