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AB10181

Sigma-Aldrich

Anti-Pumilio-2 (PUM2) Antibody

from rabbit, purified by affinity chromatography

别名:

pumilio homolog 2 (Drosophila), pumilio homolog 2, pumilio (Drosphila) homolog 2

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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物来源

rabbit

质量水平

抗体形式

affinity isolated antibody

抗体产品类型

primary antibodies

克隆

polyclonal

纯化方式

affinity chromatography

种属反应性

rhesus macaque, mouse, human

种属反应性(根据同源性预测)

chimpanzee (based on 100% sequence homology), rhesus monkey (based on 100% sequence homology), canine (based on 100% sequence homology)

技术

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI登记号

UniProt登记号

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

human ... PUM2(23369)

一般描述

PUM2 is a member of the Puf family of RNA-binding proteins, which are believed to play a role in the regulation of posttranscriptional gene expression. Members of the Puf family of RNA binding proteins function as repressors of translation when bound to the 3-untranslated region of RNA transcripts.

特异性

This antibody recognizes Pumilio-2 near the N-terminus.

免疫原

Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the N-terminus of human Pumilio-2.

应用

Detect Pumilio-2 (PUM2) using this Anti-Pumilio-2 (PUM2) Antibody validated for use in IC, IH & WB.
Immunohistochemistry Analysis: A 1:400 dilution from a previous lot detected Pumilio-2 in human testis and teratoma tissue.

Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Pumilio-2 in human and mouse embryonic stem cells.
Research Category
Stem Cell Research
Research Sub Category
RNA Metabolism & Binding Proteins

质量

Evaluated by Western Blot on mouse embryonic stem cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Pumilio-2 (PUM2) in 10 µg of mouse embryonic stem cell lysate.

目标描述

~120 kDa observed.
Non specific band may be observed at 48 kDa

外形

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Mouse embryonic stem cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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