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质量水平
产品线
Novabiochem®
方案
≥95.0% (HPLC)
≥98% (TLC)
表单
powder
反应适用性
reaction type: Fmoc solid-phase peptide synthesis
制造商/商品名称
Novabiochem®
应用
peptide synthesis
官能团
aromatic amine
储存温度
2-8°C
SMILES字符串
N(c4cc5[o][c](cc(c5cc4)CC(=O)O)=O)C(=O)OCC1c2c(cccc2)c3c1cccc3
InChI
1S/C26H19NO6/c28-24(29)11-15-12-25(30)33-23-13-16(9-10-17(15)23)27-26(31)32-14-22-20-7-3-1-5-18(20)19-6-2-4-8-21(19)22/h1-10,12-13,22H,11,14H2,(H,27,31)(H,28,29)
InChI key
RCUKLQDGAASIAX-UHFFFAOYSA-N
一般描述
Fmoc-ACA-OH is a useful tool for the SPPS of AMC fluorogenic protease substrates by Fmoc SPPS. Fmoc-ACA-OH [1] must be first loaded onto a Wang-type resin. Any unreacted linker hydroxyls must be capped using acetic anhydride. Following Fmoc removal, the first amino acid should be coupled with HATU/collidine [2]. Insertion of ACA into the middle of a peptide provides fluorogenic substrates for endopeptidases [3]. Treatment of the ACC peptide obtained after TFA cleavage with an aqueous base causes facile decarboxylation and formation of the AMC peptide.
Associated Protocols and Technical Articles
Cleavage and Deprotection Protocols for Fmoc SPPS
Chromogenic Labeling of Synthetic Peptides
Literature references
[1] J. L. Harris, et al. (2000) Proc. Natl. Acad. Sci. USA, 97, 7754.
[2] D. J. Maly, et al. (2002) J. Org. Chem., 67, 910.
[3] C. M. Salisbury, et al. (2002) J. Am. Chem. Soc., 124, 14868.
Associated Protocols and Technical Articles
Cleavage and Deprotection Protocols for Fmoc SPPS
Chromogenic Labeling of Synthetic Peptides
Literature references
[1] J. L. Harris, et al. (2000) Proc. Natl. Acad. Sci. USA, 97, 7754.
[2] D. J. Maly, et al. (2002) J. Org. Chem., 67, 910.
[3] C. M. Salisbury, et al. (2002) J. Am. Chem. Soc., 124, 14868.
分析说明
Color (visual): white to slight yellow to light red
Appearance of substance (visual): powder
Identity (IR): passes test
Purity (TLC(CMA1)): ≥ 98 %
Assay (HPLC, area%): ≥ 95.0 % (a/a)
Solubility (1 mmole in 2 ml DMF): clearly soluble
To see the solvent systems used for TLC of Novabiochem® products please click here.
Appearance of substance (visual): powder
Identity (IR): passes test
Purity (TLC(CMA1)): ≥ 98 %
Assay (HPLC, area%): ≥ 95.0 % (a/a)
Solubility (1 mmole in 2 ml DMF): clearly soluble
To see the solvent systems used for TLC of Novabiochem® products please click here.
法律信息
Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
11 - Combustible Solids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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