产品名称
D（+）-生物素, Novabiochem®
SMILES string
S1C(C2NC(=O)NC2C1)CCCCC(=O)O
InChI
1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)
InChI key
YBJHBAHKTGYVGT-UHFFFAOYSA-N
product line
Novabiochem®
form
solid
potency
>2000 mg/kg LD50, oral (Rat)
reaction suitability
reaction type: Biotinylations
manufacturer/tradename
Novabiochem®
pH
4.5 (0.1 g/L in H2O)
mp
232-233 °C
solubility
0.2 g/L
bulk density
200‑500 kg/m3
application(s)
peptide synthesis
storage temp.
2-8°C
Quality Level
Application
D(+)-生物素在SPPS中的一些最新应用：
- 蛋白质的高通量生物素化。蛋白质的位点特异性生物素化方法可用于蛋白质纯化和分析等各种应用。
- 在位点特异性生物素化的多足糖簇分子上有稳定支撑。生物素化糖簇分子的合成在糖生物学中有重要应用。
General description
D(+)-生物素是将生物素引入多肽、蛋白质和其他生物分子的最简单试剂。在SPPS中，最好使用DIPCDI/Oxyma Pure偶联。 生物素标记肽在免疫学和组织化学中有许多重要应用，如利用链霉亲和素和亲和素对生物素的高亲和力进行亲和纯化和基于FRET的流式细胞术、固相免疫测定、受体定位。[, , , ]
相关实验方案和技术文章
用于肽合成的生物素化试剂
参考文献：
[1] K. Hofmann & Y. Kiso (1976) Proc. Natl. Acad. Sci., 73, 3516.
[2] T. Buranda, et al. (1999) Cytometry, 37, 21.
[3] I. Sélo, et al. (1996) J. Immunol. Methods, 199, 127.
[4] J. Howl, et al. (1993) Eur J Biochem., 213, 711.
相关实验方案和技术文章
用于肽合成的生物素化试剂
参考文献：
[1] K. Hofmann & Y. Kiso (1976) Proc. Natl. Acad. Sci., 73, 3516.
[2] T. Buranda, et al. (1999) Cytometry, 37, 21.
[3] I. Sélo, et al. (1996) J. Immunol. Methods, 199, 127.
[4] J. Howl, et al. (1993) Eur J Biochem., 213, 711.
Legal Information
Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Peptides, antibodies, and FRET on beads in flow cytometry: A model system using fluoresceinated and biotinylated beta-endorphin
T. Buranda, et al.
Cytometry. Part A : the Journal of the International Society For Analytical Cytology, 37, 21-21 (1999)
Fluorescent and biotinylated linear peptides as selective bifunctional ligands for the V1a vasopressin receptor
J. Howl, et al.
European Journal of Biochemistry, 213, 711-711 (1993)
Synthesis of biotinylated multipodal glycoclusters on a solid support
Karskela M, et al.
European Journal of Organic Chemistry, 2012, 6594-6605 (2012)
An approach to the targeted attachment of peptides and proteins to solid supports.
K. Hofmann & Y. Kiso
Proceedings of the National Academy of Sciences, 73, 3516-3516 (1976)
High-throughput biotinylation of proteins
K Brian K, et al.
High Throughput Protein Expression and Purification, 185-198 (2009)
商品
Biotin-labelled peptides find applications in immunology and histochemistry for affinity purification and receptor localization.
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