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Merck
CN
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文件

安全信息

8.51072

Sigma-Aldrich

6-Carboxyfluorescein

Novabiochem®

别名:

6-Carboxyfluorescein, 6-FAM

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About This Item

经验公式(希尔记法):
C21H12O7
CAS号:
分子量:
376.32
MDL编号:
UNSPSC代码:
12352005
NACRES:
NA.22

质量水平

产品线

Novabiochem®

检测方案

≥95% (TLC)
≥98.0% (HPLC)

形式

powder

反应适用性

reagent type: fluorescent-labelling reagent

制造商/商品名称

Novabiochem®

应用

peptide synthesis

储存温度

2-8°C

InChI

1S/C21H12O7/c22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)21(14)16-7-10(19(24)25)1-4-13(16)20(26)28-21/h1-9,22-23H,(H,24,25)

InChI key

BZTDTCNHAFUJOG-UHFFFAOYSA-N

一般描述

This high purity fluorescent dye (λem494 nm, λex 518 nm) is supplied as a single isomer, which ensures production of labeled products of defined chemical structure, as well as greatly simplifying purification and characterization. FAM should be introduced as the last step in the synthesis to avoid formation of side-products, or protected by tritylation prior to acylation or hydrazine treatment [1].

Literature references

[1] R. Fischer, et al. (2003) Bioconjugate Chem., 14, 653.

联系

Replaces: 01-63-0113

分析说明

Color (visual): yellow to orange
Appearance of substance (visual): powder
Identity (IR): passes test
Purity (TLC (EtOAc/MeOH/H2O/DIPEA 60 : 30 : 10 : 3)): ≥ 95 %
Assay (HPLC, area%): ≥ 98.0 % (a/a)

To see the solvent systems used for TLC of Novabiochem® products please click here.

法律信息

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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Chromogenic and fluorogenic derivatives are invaluable tools for biochemistry, having numerous applications in enzymology, protein chemistry, immunology and histochemistry.

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