制造商/商品名称
Novagen®
储存条件
OK to freeze
运输
wet ice
储存温度
2-8°C
一般描述
ProteoExtract®跨膜蛋白质提取试剂盒（TM-PEK）提供了无洗涤剂的新型化学方法，可从哺乳动物细胞和组织中温和有效地提取跨膜蛋白，例如GPCR。膜蛋白中富含的级分和大蛋白复合物与酶测定、非变性和基于SDS的凝胶电泳、蛋白质印迹和免疫沉淀直接兼容。富含的级分适用于胰蛋白酶凝胶内消化后基于质谱的分析。该试剂盒包含用于蛋白质依赖性优化的两种不同提取试剂、蛋白酶抑制剂混合物和用于处理二十个样品的适当缓冲液（每个样品2-5 x 106个细胞或25-50 mg组织）。
应用
ProteoExtract®跨膜蛋白提取试剂盒已用于： 提取质膜蛋白的Na+/H+交换剂（NHE3）的蛋白质印迹分析
特点和优势
- 不需要超声、强涡流、长时间的超离心或高温孵育。
- 试剂足以处理总共40个样品。
警告
毒性：有毒（F）
其他说明
Nakamura，E.，et al. 2008.Cancer Sci.99， 1390.
法律信息
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
PROTEOEXTRACT is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Skin Irrit. 2
WGK
WGK 2
法规信息
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