71366-M
Overnight Express™ Autoinduction System 2 - Novagen
High-level protein expression without the need to monitor cell growth
别名:
Overnight Express Autoinduction System
General description
Overnight Express Autoinduction System 2
Overnight Express™ Autoinduction System 2 provides a complete, chemically defined medium for high-level protein expression with the pET System, and other IPTG-inducible expression systems, without the need to monitor cell growth. All system components have a known chemical composition, resulting in consistent product performance and the elimination of lot-to-lot variability. System 2 also features the capability to label proteins with selenomethionine (Se-Met) for downstream crystallization and x-ray diffraction studies.
The system includes six concentrated sterile solutions: OnEx solutions 1 through 6. OnEx Solutions 1, 2, and 3 are the same components included in the Overnight Express System 1 (see preceding page). OnEx Solution 4 provides trace metals to minimize growth limitations associated with mineral deficiencies and satisfy the metal requirements of metal-containing target proteins, even at high expression levels. OnEx Solution 5 is a mixture of amino acids lacking methionine. OnEx Solution 6 is a separate methionine solution. Sufficient methionine (Met) is provided to support growth of the Met auxotroph B834 while providing the ability to reduce the level of unlabeled Met for selenomethionine incorporation by Met auxotrophs.
The Overnight Express Autoinduction System 2 is extremely convenient for routine expression of proteins in multiple cultures and is ideal for high-throughput parallel analysis of protein expression, solubility screening, and purification from multiple expression clones. The tedium of preparing a defined medium from dozens of components has been simplified with the ready-to-use sterile solutions included in the Overnight Express Autoinduction System 2.
Overnight Express Autoinduction System 1
Two Overnight Express™ Autoinduction Systems (System 1 and System 2) are available, both featuring high-level protein production in the pET and other IPTG-inducible bacterial expression systems without the need to monitor cell growth. Cell mass and target protein yield are often increased several-fold as compared with conventional protocols using induction with IPTG. Also available is Overnight Express Instant TB Medium.
Overnight Express™ Autoinduction System 2 provides a complete, chemically defined medium for high-level protein expression with the pET System, and other IPTG-inducible expression systems, without the need to monitor cell growth. All system components have a known chemical composition, resulting in consistent product performance and the elimination of lot-to-lot variability. System 2 also features the capability to label proteins with selenomethionine (Se-Met) for downstream crystallization and x-ray diffraction studies.
The system includes six concentrated sterile solutions: OnEx solutions 1 through 6. OnEx Solutions 1, 2, and 3 are the same components included in the Overnight Express System 1 (see preceding page). OnEx Solution 4 provides trace metals to minimize growth limitations associated with mineral deficiencies and satisfy the metal requirements of metal-containing target proteins, even at high expression levels. OnEx Solution 5 is a mixture of amino acids lacking methionine. OnEx Solution 6 is a separate methionine solution. Sufficient methionine (Met) is provided to support growth of the Met auxotroph B834 while providing the ability to reduce the level of unlabeled Met for selenomethionine incorporation by Met auxotrophs.
The Overnight Express Autoinduction System 2 is extremely convenient for routine expression of proteins in multiple cultures and is ideal for high-throughput parallel analysis of protein expression, solubility screening, and purification from multiple expression clones. The tedium of preparing a defined medium from dozens of components has been simplified with the ready-to-use sterile solutions included in the Overnight Express Autoinduction System 2.
Overnight Express Autoinduction System 1
Two Overnight Express™ Autoinduction Systems (System 1 and System 2) are available, both featuring high-level protein production in the pET and other IPTG-inducible bacterial expression systems without the need to monitor cell growth. Cell mass and target protein yield are often increased several-fold as compared with conventional protocols using induction with IPTG. Also available is Overnight Express Instant TB Medium.
Features and Benefits
- High cell densities and protein expression levels
- Complete chemically defined medium; minimal lot-to-lot variability
- No need to monitor cell growth rate or add inducer
- Ideal for Se-Met labeling of proteins to be crystallized for x-ray diffraction studies
- Ideal for pET Expression System or other IPTG-inducible bacterial systems
- Induction of numerous expression clones simultaneously
- Compatible with cultures grown in flasks, culture tubes, and deep-well plates
- Minimal sample handling
Preparation Note
Multiple storage requirements
Other Notes
- 20 ml or 100 ml: OnEx Solution 1
- 50 ml or 125 ml: OnEx Solution 2
- 1 ml: OnEx Solution 3
- 1 ml: OnEx Solution 4
- 20 ml or 100 ml: OnEx Solution 5
- 20 ml or 100 ml: OnEx Solution 6
PRODUCT USE RESTRICTIONS - PRODUCT LABEL LICENSE
The Overnight Express™ Auto Induction Media material (Products) includes patented technology embodied therein developed at Brookhaven National Laboratory under contract with the U.S. Department of Energy. The patent applications or issued patents embodied in this Product were assigned to Brookhaven Science Associates, LLC (BSA). BSA has enabled EMD Millipore and its Affiliates to grant a non-exclusive license for use of this patented technology, including use with the enclosed Products, based upon your acceptance of the following terms:
1. These Products are to be used for non-commercial research purposes only. For the avoidance of doubt, such non-commercial research purposes include research use by academic, non-profit, and commercial entities.
2. A separate license is required for any commercial use, including the use of the enclosed Products for developing or making other materials for sale to third parties.
3. Information about licenses for commercial use of the Products may be obtained from:
Office of Technology Commercialization and Partnerships
Brookhaven National Laboratory
Building No. 490C - P.O. Box 5000
Upton, New York 11973-5000
Telephone: 631-344-4711
Fax: 631-344-3729
E-mail: pupadhya@bnl.gov
4. Information about licenses for commercial manufacture and/or sale of the Products may be obtained from:
Intellectual Property Department
EMD Millipore Corporation
28820 Single Oak Drive Temecula, CA 92590
E-mail: licensing@milliporesigma.com
5. No Products that contain this patented technology may be transferred or distributed further to third parties outside of your laboratory, unless the recipient third party receives a license from BSA and agrees to be bound by its terms.
By keeping or using the enclosed Product(s), you accept the above terms of use solely for noncommercial research purpose. If you refuse to accept the terms herein, you must return the Product(s) to EMD Millipore.
The Overnight Express™ Auto Induction Media material (Products) includes patented technology embodied therein developed at Brookhaven National Laboratory under contract with the U.S. Department of Energy. The patent applications or issued patents embodied in this Product were assigned to Brookhaven Science Associates, LLC (BSA). BSA has enabled EMD Millipore and its Affiliates to grant a non-exclusive license for use of this patented technology, including use with the enclosed Products, based upon your acceptance of the following terms:
1. These Products are to be used for non-commercial research purposes only. For the avoidance of doubt, such non-commercial research purposes include research use by academic, non-profit, and commercial entities.
2. A separate license is required for any commercial use, including the use of the enclosed Products for developing or making other materials for sale to third parties.
3. Information about licenses for commercial use of the Products may be obtained from:
Office of Technology Commercialization and Partnerships
Brookhaven National Laboratory
Building No. 490C - P.O. Box 5000
Upton, New York 11973-5000
Telephone: 631-344-4711
Fax: 631-344-3729
E-mail: pupadhya@bnl.gov
4. Information about licenses for commercial manufacture and/or sale of the Products may be obtained from:
Intellectual Property Department
EMD Millipore Corporation
28820 Single Oak Drive Temecula, CA 92590
E-mail: licensing@milliporesigma.com
5. No Products that contain this patented technology may be transferred or distributed further to third parties outside of your laboratory, unless the recipient third party receives a license from BSA and agrees to be bound by its terms.
By keeping or using the enclosed Product(s), you accept the above terms of use solely for noncommercial research purpose. If you refuse to accept the terms herein, you must return the Product(s) to EMD Millipore.
Legal Information
Express is a trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Multiple Toxicity Values, refer to MSDS
存储类别
11 - Combustible Solids
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