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形式
liquid
质量水平
制造商/商品名称
Novagen®
储存条件
OK to freeze
启动子
Promoter name: T7
selection
kanamycin
运输
wet ice
储存温度
−70°C
一般描述
pRSFDuet-1设计用于两个靶基因的共表达。该载体编码两个多克隆位点(MCS)，每个位点之前都有一个T7启动子、lac操纵子和核糖体结合位点(RBS)。该载体还携带pRSF1030复制子（也称为NTP1）、LacI基因和卡那霉素耐药标记。
应用
pRSFDuet-1 DNA - Novagen已作为载体用于：
- 克隆防御素α5 (DEFA5)的DNA片段，以进行重组表达
- 克隆Mad1597-719的编码区
- 利用N-端6X His-tag克隆家蚕Spc24 (73-162aa)的球状结构域，生成家蚕6xHis-Spc24(73-162)-Spc25(70-211) pRSF-DUET1细菌表达构建体
警告
毒性：标准处理（A）
法律信息
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Qiao Qiao et al.
Experimental cell research, 407(2), 112809-112809 (2021-09-07)
Defensins are highly conserved antimicrobial peptides, which ubiquitously expressed in different species. In addition to the functions in host defense, their aberrant expression have also been documented in cancerous tissue including breast cancer, lung caner and renal carcinoma etc. Whereas
Elyse S Fischer et al.
EMBO reports, 22(7), e52242-e52242 (2021-05-21)
During metaphase, in response to improper kinetochore-microtubule attachments, the spindle assembly checkpoint (SAC) activates the mitotic checkpoint complex (MCC), an inhibitor of the anaphase-promoting complex/cyclosome (APC/C). This process is orchestrated by the kinase Mps1, which initiates the assembly of the
Nuria Cortes-Silva et al.
Current biology : CB, 30(4), 561-572 (2020-02-08)
Accurate chromosome segregation requires assembly of the multiprotein kinetochore complex at centromeres. In most eukaryotes, kinetochore assembly is primed by the histone H3 variant CenH3 (also called CENP-A), which physically interacts with components of the inner kinetochore constitutive centromere-associated network
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