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此商品 | 320536 | 808154 | 447285 |
|---|---|---|---|
| form liquid | form liquid | form liquid | form liquid |
| assay ≥99% | assay 97% | assay - | assay 99.5% |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
| density 1.631 g/mL at 25 °C (lit.) | density 1.631 g/mL at 25 °C (lit.) | density 1.64 g/cm3 at 20 °C | density 1.631 g/mL at 25 °C (lit.) |
| anion traces chloride (Cl-): 59.0-60.2% | anion traces - | anion traces - | anion traces - |
| mp −105 °C (lit.) | mp −105 °C (lit.) | mp -104.5 °C | mp −105 °C (lit.) |
一般描述
昆虫GeneJuice<TMSYMBOL></TMSYMBOL>转染试剂是一种专有的脂质体配方，可优化Sf9昆虫细胞的最大转染效率。该试剂对细胞的毒性极低，可用于在含血清或无血清培养基中进行瞬时和稳定转染，以及将转移质粒与线性化病毒DNA共转染以生产重组杆状病毒。当使用包含立即早期杆状病毒启动子IE1的pIEx<TMSYMBOL></TMSYMBOL>和pBiEx<TMSYMBOL></TMSYMBOL>载体时，昆虫GeneJuice是Sf9昆虫细胞悬浮培养转染中大规模表达蛋白质的理想选择。
昆虫GeneJuice转染试剂以2 mg/ml混悬液的形式在20 mM MES，150 mM NaCl，pH 6.2缓冲液中提供。1 ml足以分别在10 ml悬浮培养瓶或35 mm平板中进行12或125次转染。
- 当昆虫Genejuice®与Sf9细胞系结合使用时，来自海肾荧光素酶表达与plex的原始相对光单位(RLU)为131.90，而其他试剂与Sf9一起使用时为75.726，因此相差1.7倍。
- 当昆虫Genejuice®与Sf9细胞系结合使用时，来自海肾荧光素酶表达与plex的原始相对光单位(RLU)为131.90，而其他试剂与High Five一起使用时为11.952，因此相差11倍。
- 当昆虫Genejuice®与Sf9细胞系结合使用时，来自海肾荧光素酶表达与plex的原始相对光单位(RLU)为131.90，而昆虫Genejuice与High Five一起使用时为31.897，因此相差4.1倍。
昆虫GeneJuice转染试剂以2 mg/ml混悬液的形式在20 mM MES，150 mM NaCl，pH 6.2缓冲液中提供。1 ml足以分别在10 ml悬浮培养瓶或35 mm平板中进行12或125次转染。
警告
毒性：标准处理（A）
法律信息
GeneJuice是EMD Biosciences，Inc.，Madison，WI USA的注册商标
GENEJUICE is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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