推荐产品
制造商/商品名称
Novagen®
储存条件
OK to freeze
储存温度
10-30°C
1 of 4
此商品 | E045 | E030 | E037 |
|---|---|---|---|
| format single component solution | format single component solution | format single component solution | format single component solution |
| form liquid | form liquid | form liquid | form liquid |
| Quality Level 300 | Quality Level 300 | Quality Level 300 | Quality Level - |
| grade certified reference material | grade certified reference material | grade certified reference material | grade certified reference material |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
一般描述
Rapid purification of PCR products for downstream procedures
The SpinPrep<TMSYMBOL></TMSYMBOL> PCR Clean-Up Kit is designedfor the rapid purification of DNA amplifiedin PCR. The 10-minute procedure involvesaddition of a binding buffer followed byadsorption of the DNA to a silica membrane ina spin column format. Following a wash step,the DNA is eluted in low-salt buffer. This kitremoves DNA polymerases, dNTPs, salts, and> 99% of primers so that they do not interferewith downstream applications such as cloning,sequencing, or labeling. PCR products from100 bp to > 12,000 bp can be cleaned up, withrecoveries of amplified DNA in the range of60-90% under standard conditions.
Column binding capacity: up to 6 g
PCR volume: 100 l/rxn
Typical recovery: 6090%
Size range: 100 bp to > 12,000 bp
Time required:< 10 min
Column binding capacity: up to 6 g
PCR volume: 100 l/rxn
Typical recovery: 6090%
Size range: 100 bp to > 12,000 bp
Time required:< 10 min
组分
•82 mlSpinPrep Bind Buffer
•27 mlSpinPrep Wash Buffer
•10 mlSpinPrep Elute Buffer
•100SpinPrep Filters
•100Receiver Tubes
•100Eluate Receiver Tubes
•27 mlSpinPrep Wash Buffer
•10 mlSpinPrep Elute Buffer
•100SpinPrep Filters
•100Receiver Tubes
•100Eluate Receiver Tubes
警告
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
法律信息
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语：
Warning
危险分类
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
储存分类代码
10 - Combustible liquids
WGK
WGK 1
Yong H Park et al.
Investigative ophthalmology & visual science, 57(2), 508-526 (2016-02-13)
The α-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid (AMPA) receptors (AMPAR) subunits can be posttranscriptionally modified by alternative splicing forming flip and flop isoforms. We determined if an ischemia-like insult to retinal ganglion cells (RGCs) increases AMPAR susceptibility to s-AMPA-mediated excitotoxicity through changes in posttranscriptional
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