制造商/商品名称
Novagen®
储存条件
OK to freeze
运输
wet ice
一般描述
BugBuster®Ni-NTA HIS-BIND®纯化试剂盒用于蛋白质纯化。Ni-NTA HIS-BIND®树脂是一种高性能的Ni2+带电琼脂糖，用于快速一步纯化含多组氨酸标签序列的蛋白质。
应用
BugBuster®Ni-NTA HIS-BIND®纯化试剂盒已用于His标记蛋白的纯化，如海藻糖-6-磷酸磷酸酶(TPP)、Ras(WT)蛋白和CsgA（主要curlin亚基）蛋白。
组分
•2 × 100 mlBugBuster 蛋白提取试剂
•10,000 U苯甲酸酯酶核酸酶，纯度> 90％
•10 mlNi-NTA His•结合树脂
•pkg/4色谱柱
•10,000 U苯甲酸酯酶核酸酶，纯度> 90％
•10 mlNi-NTA His•结合树脂
•pkg/4色谱柱
警告
毒性：多个毒性值，请参阅 MSDS（O）
其他说明
由于该运输中有害物质的性质，您的订单可能需要支付额外的运输费用。某些尺寸的产品可免除其他有害材料的运输费用。请与您当地的销售办事处联系，以获取有关这些费用的更多信息。
法律信息
BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语：
Danger
危险声明
危险分类
Flam. Liq. 2
储存分类代码
3 - Flammable liquids
WGK
WGK 3
Chae-Seok Lim et al.
Small (Weinheim an der Bergstrasse, Germany), 13(40) (2017-08-16)
Intermolecular interactions dominate the behavior of signal transduction in various physiological and pathological cell processes, yet assessing these interactions remains a challenging task. Here, this study reports a single-molecule force spectroscopic method that enables functional delineation of two interaction sites
Sagar Lahiri et al.
Journal of cellular physiology, 229(9), 1245-1255 (2014-01-22)
Trehalose-6-phosphate phosphatase (TPP) catalyzes the final step in the biosynthesis of the anti-stress sugar trehalose. An 82 kDa TPP enzyme was isolated from Candida utilis with 61% yield and 43-fold purification. The protein sequence, determined by N-terminal sequencing and MALDI-TOF analysis
Sarah A Tursi et al.
PLoS pathogens, 13(4), e1006315-e1006315 (2017-04-15)
Bacterial biofilms are associated with numerous human infections. The predominant protein expressed in enteric biofilms is the amyloid curli, which forms highly immunogenic complexes with DNA. Infection with curli-expressing bacteria or systemic exposure to purified curli-DNA complexes triggers autoimmunity via
商品
This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门