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Merck
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主要文件

70751

Millipore

BugBuster® Ni-NTA His•Bind®纯化试剂盒

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4 L
¥1,181.00

¥1,181.00


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选择尺寸

变更视图
4 L
¥1,181.00

About This Item

UNSPSC代码:
41106500
NACRES:
NA.56

¥1,181.00


请联系客服了解存货情况

制造商/商品名称

Novagen®

储存条件

OK to freeze

运输

wet ice

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1 of 4

此商品
T7527T4415T9650
form

solution

form

powder blend

form

solution

form

solution

pH

8.2-8.4 (25 °C)

pH

8.1-8.5 (5 ×)

pH

-

pH

-

impurities

DNase and RNase, none detected

impurities

DNase and RNase, none detected

impurities

DNase and RNase, none detected

impurities

-

sterility

0.2 μm filtered

sterility

-

sterility

-

sterility

non-sterile; 0.2 μm filtered

一般描述

BugBuster®Ni-NTA HIS-BIND®纯化试剂盒用于蛋白质纯化。Ni-NTA HIS-BIND®树脂是一种高性能的Ni2+带电琼脂糖,用于快速一步纯化含多组氨酸标签序列的蛋白质。

应用

BugBuster®Ni-NTA HIS-BIND®纯化试剂盒已用于His标记蛋白的纯化,如海藻糖-6-磷酸磷酸酶(TPP)[1]、Ras(WT)蛋白[2]和CsgA(主要curlin亚基)蛋白。[3]

组分

•2 × 100 mlBugBuster 蛋白提取试剂

•10,000 U苯甲酸酯酶核酸酶,纯度> 90%

•10 mlNi-NTA His•结合树脂

•pkg/4色谱柱

警告

毒性:多个毒性值,请参阅 MSDS(O)

其他说明

由于该运输中有害物质的性质,您的订单可能需要支付额外的运输费用。某些尺寸的产品可免除其他有害材料的运输费用。请与您当地的销售办事处联系,以获取有关这些费用的更多信息。

法律信息

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

象形图

Flame

警示用语:

Danger

危险声明

危险分类

Flam. Liq. 2

储存分类代码

3 - Flammable liquids

WGK

WGK 3

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    Sagar Lahiri et al.
    Journal of cellular physiology, 229(9), 1245-1255 (2014-01-22)
    Trehalose-6-phosphate phosphatase (TPP) catalyzes the final step in the biosynthesis of the anti-stress sugar trehalose. An 82 kDa TPP enzyme was isolated from Candida utilis with 61% yield and 43-fold purification. The protein sequence, determined by N-terminal sequencing and MALDI-TOF analysis
    Chae-Seok Lim et al.
    Small (Weinheim an der Bergstrasse, Germany), 13(40) (2017-08-16)
    Intermolecular interactions dominate the behavior of signal transduction in various physiological and pathological cell processes, yet assessing these interactions remains a challenging task. Here, this study reports a single-molecule force spectroscopic method that enables functional delineation of two interaction sites
    Sarah A Tursi et al.
    PLoS pathogens, 13(4), e1006315-e1006315 (2017-04-15)
    Bacterial biofilms are associated with numerous human infections. The predominant protein expressed in enteric biofilms is the amyloid curli, which forms highly immunogenic complexes with DNA. Infection with curli-expressing bacteria or systemic exposure to purified curli-DNA complexes triggers autoimmunity via

    商品

    This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.

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