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一般描述
His•Bind® purification kit includes pre-tested buffers specifically designed for IDA-based His•Bind Resin, enabling convenient and rapid one-step purification of proteins by metal affinity chromatography. It uses the His•Tag®/His•Bind® technology, that leverages the affinity between neighboring histidine in the His•Tag sequence and immobilized metal ions (such as Ni2+ or Co2+).
应用
His•Bind® Purification Kit has been used for: the purification of N-terminal 6-histidine-tagged aldehyde dehydrogenase isozyme, ALDH1A1 under nondenaturing conditions by nickel-NTA affinity chromatography, the purification of virus binding protein specific for Poliovirus Type 1, the purification of recombinant oE7, huhsp70 and oE7/huhsp70 proteins, to study the effective antitumor efficacy of human hsp70 and HPV16 oE7 fusion protein vaccine, the purification of recombinant exFPR1, MTBHsp70 and MTBHsp70-exFPR1 fusion proteins to study their role in cervical cancer immunotherapy
特点和优势
- Includes His•Bind® Resin, His•Bind® buffer kit and chromatography columns.
- Solutions are included for Ni2+ charging, binding, washing, and elution.
- Columns are pre-packed with 1.25 ml bed volume of Ni2+-charged His•Bind Resin.
组分
•10 ml组氨酸•结合树脂
•1个组氨酸•结合缓冲试剂盒
•每包装4个色谱柱
•1个组氨酸•结合缓冲试剂盒
•每包装4个色谱柱
警告
毒性:多个毒性值,请参阅MSDS(O)
其他说明
由于该运输中有害物质的性质,您的订单可能需要支付额外的运输费用。某些尺寸的产品可免除其他有害材料的运输费用。请与您当地的销售办事处联系,以获取有关这些费用的更多信息。
法律信息
HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语:
Danger
危险分类
Aquatic Chronic 2 - Carc. 1A Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1C - Skin Sens. 1 - STOT RE 1 - STOT RE 2 Inhalation
靶器官
respiratory tract irritation
WGK
WGK 3
闪点(°F)
86.0 °F
闪点(°C)
30 °C
法规信息
危险化学品
MTBHsp70-exFPR1-pulsed Dendritic Cells Enhance the Immune Response against Cervical Cancer
Journal of Cancer, 10(25), 6364?6373-6364?6373 (2019)
Human hsp70 and HPV16 oE7 fusion protein vaccine induces an effective antitumor efficacy
Oncology Reports, 30(1), 407-412 (2013)
Construction of a Cloning System for the Mass Production of a Virus-Binding Protein Specific for Poliovirus Type 1
Applied and Environmental Microbiology, 71(5) (2005)
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