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一般描述
S蛋白琼脂糖可特异性保留S•标签 融合的蛋白。结合可以以柱的形式或批处理模式进行。使用稍微变性的条件或使用特定的蛋白酶(例如凝血酶或肠激酶)即可对适当的重组蛋白进行洗脱。S-蛋白琼脂糖的载量会发生变化,其数值基于所给定靶蛋白的大小和折叠特性。
它包含 15 个氨基酸的序列 Lys-Glu-Thr-Ala-Ala-Ala-Lys-Phe-Glu-Arg-Gln-His-Met-Asp-Ser (KETAAAKFERQHMDS)。
应用
S 蛋白琼脂糖已用于纯化 S 重组标记融合蛋白。
警告
毒性:标准处理(A)
法律信息
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Charles F Ericson et al.
eLife, 8 (2019-09-19)
The swimming larvae of many marine animals identify a location on the sea floor to undergo metamorphosis based on the presence of specific bacteria. Although this microbe-animal interaction is critical for the life cycles of diverse marine animals, what types
Qian Li et al.
Journal of virology, 93(5) (2018-12-14)
Human herpesvirus 8 (HHV-8) viral interleukin-6 (vIL-6) localizes largely to the endoplasmic reticulum (ER) and here associates functionally with both the gp130 signal transducer and the novel ER membrane protein vitamin K epoxide reductase complex subunit 1 variant-2 (VKORC1v2). The
Binquan Huang et al.
Archives of biochemistry and biophysics, 596, 63-72 (2016-03-05)
Starch synthases SSI, SSII, and SSIII function in assembling the amylopectin component of starch, but their specific roles and means of coordination are not fully understood. Genetic analyses indicate regulatory interactions among SS classes, and physical interactions among them are
Xiaoting Liang et al.
Theranostics, 10(17), 7747-7757 (2020-07-21)
Background: Rab22a-NeoF1 fusion gene containing the 1-38aa of Rab22a (Rab22a1-38) plays a decisive role in driving tumor metastasis by activating RhoA via binding to SmgGDS607. However, its intercellular regulation remains unknown. Methods: The Lys7 (K7) acetylation of Rab22a-NeoF1 was initially
Toshiki Takahashi et al.
EMBO reports, 20(4) (2019-02-26)
Rab family small GTPases are master regulators of distinct steps of intracellular vesicle trafficking in eukaryotic cells. GDP-bound cytoplasmic forms of Rab proteins are prone to aggregation due to the exposure of hydrophobic groups but the machinery that determines the
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