推荐产品
抗体形式
purified antibody
质量水平
抗体产品类型
primary antibodies
制造商/商品名称
Novagen®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
unmodified
一般描述
在含有0.1 M三甘氨酸、0.15 M NaCl、pH 7.4、0.05%叠氮化钠和50%甘油的缓冲液中作为纯化兔多克隆抗体提供。
用重组Cre-His进行蛋白质印迹分析。
蛋白质印迹分析：代表性批次数据。用目录号69050-3，抗Cre（1:10000稀释）探测以20 ng/泳道（泳道 1）和10 ng/泳道（泳道2）加载的重组Cre-His。使用与HRP偶联的驴抗兔二抗和化学发光检测系统对蛋白质进行可视化检查。箭头表示Cre（~39 kDa）。
用重组Cre-His进行蛋白质印迹分析。
蛋白质印迹分析：代表性批次数据。用目录号69050-3，抗Cre（1:10000稀释）探测以20 ng/泳道（泳道 1）和10 ng/泳道（泳道2）加载的重组Cre-His。使用与HRP偶联的驴抗兔二抗和化学发光检测系统对蛋白质进行可视化检查。箭头表示Cre（~39 kDa）。
细胞和细胞裂解物中Cre重组酶的特异、灵敏检测
重组酶cre (UniProt：P06956；也称为Cre）由在大肠埃希菌噬菌体P1（噬菌体P1）中的Cre基因编码（基因ID：2777477）。Cre（引起与环化重组酶的重组）是′噬菌体′整合酶家族的38 kDa DNA重组酶，来源于P1噬菌体。它催化位点特异性重组，并对P1 DNA中发现的34 bp DNA序列基序loxP（X-over P1位点）具有高度特异性。loxP位点由两组13 bp回文序列（ATAACTTCGTATA-NNNTANNN-tatacgaagtat）之间的可变不对称8-bp序列组成。两个cre重组酶蛋白，每个结合到loxP位点的两个13 bp回文序列中的一个，形成二聚体。然后，该二聚体与另一个loxP位点上的第二个二聚体结合形成四聚体。由四聚体连接的两个位点在方向上平行，双链DNA在两个loxP位点均被切割。然后用DNA连接酶重新连接这些链。由于Cre基因和loxP位点不是大多数物种所固有的，它们通常被引入靶细胞或组织中，并被用作一种手段来精确控制体外培养细胞和体内动物中的基因表达。
警告
毒性：标准处理（A）
法律信息
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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