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Merck
CN
所有图片(1)

文件

69026

Millipore

T7•Tag® Antibody Agarose

别名:

T7 Tag Antibody

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About This Item

UNSPSC代码:
41106500
NACRES:
NA.56

形式

liquid

质量水平

制造商/商品名称

Novagen®

储存条件

do not freeze

运输

wet ice

储存温度

2-8°C

一般描述

The T7•Tag Antibody Agarose is designed for rapid immunoaffinity purification of target proteins that carry the T7•Tag sequence (i.e., the amino terminal 11 aa of the T7 gene 10 protein). Purification is based on binding target proteins to T7•Tag monoclonal antibody that is covalently coupled to cross-linked agarose beads, washing away unbound proteins, and eluting at pH 2.2. Capacity will vary somewhat between different target proteins, but the beads are standardized to bind a minimum of 300 µg T7•Tag β-galactosidase per milliliter of settled resin. The beads can be used in either batch or column methods and can be recycled a minimum of five times without loss of binding activity.

警告

Toxicity: Standard Handling (A)

法律信息

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
T7-Tag is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Satoshi Hirano et al.
Autophagy, 12(2), 312-326 (2016-02-24)
MAP1LC3/LC3 (a mammalian ortholog family of yeast Atg8) is a ubiquitin-like protein that is essential for autophagosome formation. LC3 is conjugated to phosphatidylethanolamine on phagophores and ends up distributed both inside and outside the autophagosome membrane. One of the well-known
Subhashini Sadasivam et al.
Genes & development, 26(5), 474-489 (2012-03-07)
Cell cycle progression is dependent on two major waves of gene expression. Early cell cycle gene expression occurs during G1/S to generate factors required for DNA replication, while late cell cycle gene expression begins during G2 to prepare for mitosis.
Sara R Heras et al.
Nature structural & molecular biology, 20(10), 1173-1181 (2013-09-03)
More than half of the human genome is made of transposable elements whose ongoing mobilization is a driving force in genetic diversity; however, little is known about how the host regulates their activity. Here, we show that the Microprocessor (Drosha-DGCR8)
Koutaro Ishibashi et al.
Autophagy, 7(12), 1500-1513 (2011-11-16)
A large protein complex consisting of Atg5, Atg12 and Atg16L1 has recently been shown to be essential for the elongation of isolation membranes (also called phagophores) during mammalian autophagy. However, the precise function and regulation of the Atg12–5-16L1 complex has
Andres López-Perrote et al.
eLife, 9 (2020-11-19)
Nonsense-mediated mRNA decay (NMD) is a surveillance pathway that degrades aberrant mRNAs and also regulates the expression of a wide range of physiological transcripts. RUVBL1 and RUVBL2 AAA-ATPases form an hetero-hexameric ring that is part of several macromolecular complexes such

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