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Merck
CN
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主要文件

662057

Sigma-Aldrich

Ubiquitin, GST-Fusion, Recombinant, E. coli

别名:

GST-Ub, no change

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About This Item

UNSPSC代码:
12352202
NACRES:
NA.77

方案

≥95% (SDS-PAGE)

质量水平

表单

liquid

制造商/商品名称

Calbiochem®

储存条件

OK to freeze
avoid repeated freeze/thaw cycles

技术

cell based assay: suitable

NCBI登记号

运输

wet ice

储存温度

−70°C

一般描述

Research area: Cancer

Recombinant ubiquitin fused at the N-terminus to GST and expressed in E. coli. Useful for glutathione affinity purification of ubiquitinated molecules. Can also be used for immunodetection of conjugates using GST antibodies. Facilitates the visualization of polyUb chains due to larger ladder intervals.

应用

Ubiquitin, GST-Fusion, Recombinant, E. coli has been used for in vivo and in vitro ubiquitination assays, to study the role of FBXL2-Grp94-EGFR axis in non-small cell lung cancer (NSCLC) development and the role of F-box protein 3 (FBXO3) in promotion of breast cancer metastasis.

生化/生理作用

The ubiquitin (Ub) system plays a crucial role in protein homeostasis by maintaining protein balance in pathways related to DNA repair, cell cycle progression, apoptosis (programmed cell death), receptor-mediated endocytosis, and signal transduction. Imbalances or dysfunctions within this system can lead to pathological conditions. Apart from this, disruptions in ubiquitin pathways can also contribute to the development of human diseases, including neurodegenerative conditions and tumors.

包装

Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

外形

In 100 mM NaCl, 50 mM HEPES, pH 8.0.

重悬

Following initial thaw, aliquot and freeze (-70°C).

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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访问文档库

Ubiquitin networks in cancer
Kirkin V and Dikic I
Current Opinion in Genetics & Development, 21(1), 21-28 (2011)
Ubiquitin
Kona F.R, et al.
Brenner and Rector's The Kidney E-book, 242-245 (2013)
Metalloregulation of Protein Clearance: New Therapeutic Avenues for Neurodegenerative Diseases
Munoz ET, et al.
Abstr. Soc. Neurosci (2017)
Mengmeng Niu et al.
PLoS biology, 19(2), e3001113-e3001113 (2021-02-25)
Transforming growth factor-β (TGF-β) signaling plays a critical role in promoting epithelial-to-mesenchymal transition (EMT), cell migration, invasion, and tumor metastasis. ΔNp63α, the major isoform of p63 protein expressed in epithelial cells, is a key transcriptional regulator of cell adhesion program
Mengmeng Niu et al.
Nature communications, 12(1), 5919-5919 (2021-10-13)
Abnormal activation of epidermal growth factor receptor (EGFR) drives non-small cell lung cancer (NSCLC) development. EGFR mutations-mediated resistance to tyrosine-kinase inhibitors (TKIs) is a major hurdle for NSCLC treatment. Here, we show that F-box protein FBXL2 targets EGFR and EGFR

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