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生物来源
mouse
质量水平
重组
expressed in E. coli
方案
≥97% (SDS-PAGE)
表单
lyophilized
分子量
17,000 g/mol
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
技术
cell based assay: suitable
杂质
≤100 pg/μg Endotoxin (pg/μg TNF-α)
运输
ambient
储存温度
−70°C
基因信息
mouse ... Tnf(21926)
一般描述
Reasearch area: Apoptosis
Recombinant mouse tumor necrosis factor-α (TNF-α), expressed in E. coli, is known for its direct tumoricidal capabilities. This inflammatory cytokine triggers a cascade of other cytokines and factors constituting the immune system′s response to infections and cancer. Additionally, it has been reported to influence changes in bone metabolism during inflammation. (TNF-α), a homotrimer protein, occurs in a soluble and transmembrane form.
Recombinant mouse tumor necrosis factor-α (TNF-α), expressed in E. coli, is known for its direct tumoricidal capabilities. This inflammatory cytokine triggers a cascade of other cytokines and factors constituting the immune system′s response to infections and cancer. Additionally, it has been reported to influence changes in bone metabolism during inflammation. (TNF-α), a homotrimer protein, occurs in a soluble and transmembrane form.
应用
TNF-α, Mouse, Recombinant, E. coli has been used to study TNF-signaling to insulin gene expression in insulin-producing cells.
生化/生理作用
TNF-α, a cytokine, interacts with two distinct receptors, initiating signal transduction pathways that result in cellular responses, such as cell survival, differentiation, and proliferation. It regulates inflammatory responses and activates inflammatory molecules, including other cytokines and chemokines. Transmembrane TNF-α binds to TNFR1 and TNFR2 and facilitates inflammation and cell death. However, excessive or inappropriate activation of TNF-α signaling is linked to chronic inflammation and may lead to pathological complications, including autoimmune diseases. Rheumatoid arthritis (RA), inflammatory bowel disease (IBD), psoriatic arthritis (PsA), psoriasis (PS), and noninfectious uveitis (NIU) are few conditions.
Additionally, TNF-α is associated with insulin signaling inhibition. Its removal ameliorates insulin sensitivity. The activity of the Jun N-terminal kinase (JNK) signaling pathway, which is abnormally elevated in obesity, is strongly activated by both TNF-α.
ED₅₀ = 20-50 pg/ml as measured in a cytotoxic assay using the TNF-α susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)
Additionally, TNF-α is associated with insulin signaling inhibition. Its removal ameliorates insulin sensitivity. The activity of the Jun N-terminal kinase (JNK) signaling pathway, which is abnormally elevated in obesity, is strongly activated by both TNF-α.
ED₅₀ = 20-50 pg/ml as measured in a cytotoxic assay using the TNF-α susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)
警告
Toxicity: Standard Handling (A)
外形
Lyophilized from sterile-filtered PBS, 50 µg BSA/µg TNF-α.
重悬
Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
Reconstitute to a concentration ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.
其他说明
Scharla, S.H., et al. 1994. Eur. J. Endocrinol. 131, 293.
Eck, M.J., et al. 1992. J. Biol. Chem. 267, 2119.
Eck, M.J., et al. 1992. J. Biol. Chem. 267, 2119.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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