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质量水平
形式
lyophilized
制造商/商品名称
Calbiochem®
存货情况
not available in, Canada
储存条件
do not freeze
溶解性
sterile distilled water: soluble
储存温度
2-8°C
一般描述
A high molecular weight glucosyltransferase that inhibits small GTPases, such as Rho, Rac, and Cdc42 by glucosylation of a threonine residue. This results in the shutdown of signal transduction cascades, leading to depolymerization of the cytoskeleton, gene transcription of a number of stress-activated protein kinases (SAPKs), and a decrease in the synthesis of phosphatidylinositol 4,5-bisphosphate. May also block serotonin release, and stimulate the production of phospholipases C and D. Due to its ability to affect depolymerization of the cytoskeleton, toxin B has been shown to inhibit biphasic muscle contraction. Unlike Exoenzyme C3 (Cat. No. 341208), this toxin does not require assistance to permeabilize cells.
A high molecular weight glucosyltransferase that inhibits small GTPases, such as Rho, Rac, and Cdc42, by glucosylation of a threonine residue.
A high molecular weight glucosyltransferase that inhibits small GTPases, such as Rho, Rac, and Cdc42, by glucosylation of a threonine residue. This results in the shutdown of signal transduction cascades, leading to depolymerization of the cytoskeleton, gene transcription of a number of stress-activated protein kinases (SAPKs), and a decrease in the synthesis of phosphatidylinositiol 4,5-bisphosphate. May also block serotonin release, and stimulate the production of phospholipases C and D. Due to its ability to affect depolymerization of the cytoskeleton, toxin B has been shown to inhibit biphasic muscle contraction. Unlike Exoenzyme C3 (Cat. No. 341208), this toxin does not require assistance to permeabilize cells.
生化/生理作用
Cytotoxic as determined in a cell rounding assay using human foreskin cells.
Primary Target
Small GTPases, such as Rho, Rac, and Cdc42
Small GTPases, such as Rho, Rac, and Cdc42
Product does not compete with ATP.
警告
Toxicity: Irritant (B)
外形
Lyophilized from 50 mM Tris, 50 mM NaCl, 0.1% trehalose, pH 7.5.
重悬
Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 2 weeks at 4°C. Toxin B activity will decrease after 2 weeks at 4°C following reconstitution.
Please see vial label for lot-specific reconstitution volume.
分析说明
major band at >200 kDa
其他说明
Mackay, D.J. and Hall, A. 1998. J. Biol. Chem. 273, 20685.
Prepens, U., et al. 1996. J. Biol. Chem. 271, 7324.
Schmidt, M., et al. 1996. Eur. J. Biochem. 240, 707.
Wilkins, T.D. and Lyerly, D.M. 1996. Trends Microbiol. 4, 49.
Prepens, U., et al. 1996. J. Biol. Chem. 271, 7324.
Schmidt, M., et al. 1996. Eur. J. Biochem. 240, 707.
Wilkins, T.D. and Lyerly, D.M. 1996. Trends Microbiol. 4, 49.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语:
Danger
危险声明
危险分类
Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Acute Tox. 3 Dermal
WGK
WGK 3
法规信息
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