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一般描述
一种即用型稳定、中性形式的TCEP盐酸盐（目录号580560），一种二硫化物还原剂。
一种即用型稳定、中性形式的常用二硫化物还原剂TCEP，盐酸盐（目录号580560)0）。用1 ml H2O复溶每个小瓶，以获得500 mM溶液。
应用
- Förster Resonance Energy Transfer Assay for Investigating the Reactivity of Thioesters in Biochemistry and Native Chemical Ligation.: This study utilizes TCEP in a FRET assay to explore thioester reactivity, providing insights into biochemical reactions and peptide synthesis applications (Gless et al., 2023).
- Two-Dimensional Capillary Zone Electrophoresis-Mass Spectrometry: Intact mAb Charge Variant Separation Followed by Peptide Level Analysis Using In-Capillary Digestion.: TCEP is employed for in-capillary digestion to enhance protein separation and analysis, improving the characterization of monoclonal antibodies (Schlecht et al., 2023).
- Silica Nanoparticle-Loaded Thermoresponsive Block Copolymer Vesicles: A New Post-Polymerization Encapsulation Strategy and Thermally Triggered Release.: TCEP is used in the synthesis of block copolymer vesicles, facilitating the encapsulation and controlled release of nanoparticles for biomedical applications (Czajka et al., 2022).
- Thiol Catalysis of Selenosulfide Bond Cleavage by a Triarylphosphine.: This research demonstrates the use of TCEP in catalyzing selenosulfide bond cleavage, contributing to the understanding of thiol-based reduction mechanisms (Firstova et al., 2022).
- A Novel PNGase Rc for Improved Protein N-Deglycosylation in Bioanalytics and Hydrogen-Deuterium Exchange Coupled With Mass Spectrometry Epitope Mapping Under Challenging Conditions.: TCEP is highlighted for its role in improving protein deglycosylation and mass spectrometry analysis, enhancing bioanalytical techniques (Gramlich et al., 2022).
包装
用惰性气体包装
警告
毒性：腐蚀性（L）
重悬
复溶后，等分，并用惰性气体冲洗并冷冻（-20°C）。贮备溶液在-20°C下可稳定保存至多1个月。
用1 ml水复溶以获得500 mM溶液。
其他说明
Getz, E.B., et al. 1999.Anal.Biochem.273, 73.
Han, J.C. and Han, G.Y.1994.Anal.Biochem.220, 5.
Burns, J.A., et al. 1991.J. Org.Chem.56, 2648.
Han, J.C. and Han, G.Y.1994.Anal.Biochem.220, 5.
Burns, J.A., et al. 1991.J. Org.Chem.56, 2648.
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法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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