形式
liquid
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
desiccated
技术
electrophoresis: suitable
异质活性
DNase, RNase, protease, none detected
运输
ambient
储存温度
15-25°C
相关类别
一般描述
三乙酸和乙二胺四乙酸(TAE)缓冲液由三(2-氨基-2-[羟甲基]-1,3-丙二醇)碱、乙酸和乙二胺四乙酸(EDTA)组成。这是核酸电泳中最常用的运行缓冲液之一。TAE缓冲液提高了大DNA片段的分离度或分辨率。TAE是一种标准缓冲液,主要用于板凝胶中DNA和RNA的琼脂糖凝胶电泳。TAE是每个生物化学和分子生物学实验室常用的缓冲液之一。
应用
TAE缓冲液(10倍),分子生物学级 - Calbiochem已在1X浓度下用于制备琼脂糖凝胶并作为凝胶电泳的运行缓冲液。
警告
毒性:刺激性(B)
制备说明
用H₂O稀释1:10,制备1X溶液。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语:
Warning
危险声明
危险分类
Eye Irrit. 2
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Nature protocols, 13(5), 899-914 (2018-04-07)
Cpf1, a CRISPR endonuclease discovered in Prevotella and Francisella 1 bacteria, offers an alternative platform for CRISPR-based genome editing beyond the commonly used CRISPR-Cas9 system originally discovered in Streptococcus pyogenes. This protocol enables the design of engineered CRISPR-Cpf1 components, both
Modification of gel architecture and TBE/TAE buffer composition to minimize heating during agarose gel electrophoresis
Analytical Biochemistry, 454, 44-52 (2014)
Analytical biochemistry, 454, 44-52 (2014-03-19)
Agarose gel electrophoresis of DNA and RNA is routinely performed using buffers containing either Tris, acetate, and EDTA (TAE) or Tris, borate, and EDTA (TBE). Gels are run at a low, constant voltage (∼10 V/cm) to minimize current and asymmetric
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